MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02448nam a22003257a 4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
G94667 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20220205062607.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
121211s ||||f| 0 p|p||0|| | |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| International Standard Book Number |
978-979-1159-41-8 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
ShivNarayan Sharma |
| Affiliation |
Proceedings of the Asian Regional Maize Workshop, 10; Makassar (Indonesia); 20-23 Oct 2008 |
| 245 00 - TITLE STATEMENT |
|---|
| Title |
In silico mining of EST-SSRs from putative drought stress-responsive candidate genes and polymorphism survey in selected maize inbred lines |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
Mexico, DF (Mexico) |
| Name of publisher, distributor, etc. |
CIMMYT : |
| Date of publication, distribution, etc. |
2010 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
p. 304-308 |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Maize production and productivity is severely affected worldwide by water deficit stress, particularly in countries like India where nearly 80 percent of maize is cultivated under rainfed conditions. We have undertaken in silico mining of microsatellite/SSR markers from the EST sequences of putative drought stress-responsive candidate genes in maize. The EST sequences from the NCBI database were processed and clustered to reduce redundancy to develop consensus sequences. The consensus sequences were searched for SSR motifs. In total, 930 di- to hexa-repeat motif SSR markers were identified, including 132 from EST-SSR sequences that are homologs of functionally characterized genes involved in dehydration responses. The mined data was validated using tBLASTn and tBLASTx searches of the retrieved regions against the databases at NCBI, built inside the MaizeEST project. Validated sequences were used for designing primers (through PBC Bioinformatics) for an initial set of 132 ESTSSRs. These SSRs were used for analysis of SSR polymorphisms in a set of 24 maize inbred lines with significant differences in responses to drought stress in India. The genic SSRs, identified based on putative drought-stress responsive genes in maize, can be potentially for various purposes, including genetic diversity analysis, comparative mapping and marker-assisted selection (MAS). |
| 536 ## - FUNDING INFORMATION NOTE |
|---|
| Text of note |
Global Maize Program |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
English |
| 591 ## - CATALOGING NOTES |
|---|
| Affiliation |
No CIMMYT affiliation |
| 594 ## - STAFFID |
|---|
| StaffID |
INT3057 |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Bioinformatics |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
1080 |
| Topical term or geographic name as entry element |
Drought |
| Miscellaneous information |
AGROVOC |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
EST-SSRs |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
functional diversity |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Maize |
| Miscellaneous information |
AGROVOC |
| Source of heading or term |
|
| 9 (RLIN) |
1173 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Zaidi, P.H.|Azrai, M.|Pixley, K.V. |
| Relator term |
eds. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Prasanna, B.M. |
| Miscellaneous information |
Global Maize Program |
| Field link and sequence number |
INT3057 |
| 9 (RLIN) |
887 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Conference proceedings |
| Source of classification or shelving scheme |
Dewey Decimal Classification |