Establishing genomics-assisted breeding foundation in chickpea for enhancing crop productivity in Africa and Asia (Record no. 8023)
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fixed length control field | 03633nam a22002177a 4500 |
001 - CONTROL NUMBER | |
control field | 8023 |
003 - CONTROL NUMBER IDENTIFIER | |
control field | MX-TxCIM |
005 - DATE AND TIME OF LATEST TRANSACTION | |
control field | 20240808230555.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
fixed length control field | 121211s ||||f| 0 p|p||0|| | |
040 ## - CATALOGING SOURCE | |
Original cataloging agency | MX-TxCIM |
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | CIS-6173 |
100 1# - MAIN ENTRY--PERSONAL NAME | |
Personal name | Varshney, R.K. |
Affiliation | International Symposium on Genomics of Plant Genetic Resources, II; Bologna (Italy); 24-27 Apr 2010 |
245 00 - TITLE STATEMENT | |
Title | Establishing genomics-assisted breeding foundation in chickpea for enhancing crop productivity in Africa and Asia |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
Date of publication, distribution, etc. | 2010 |
300 ## - PHYSICAL DESCRIPTION | |
Extent | p. 53 |
500 ## - GENERAL NOTE | |
General note | Abstract only |
520 ## - SUMMARY, ETC. | |
Summary, etc. | Chickpea, an important food legume is generally grown in marginal environments of semi-arid regions of Asia and Africa|where the crop production is heavily challenged by abiotic stresses such as terminal drought and biotic factors like|legume pod borer (Helicoverpa armigera), fusarium wilt and ascochyta blight. With an objective to tackle these|production constraints through molecular breeding, in collaboration with several partners around the world, significant|genomic resources have been developed recently (http://www.icrisat.org/gt-bt/ICGGC/homepage.htm). For instance,|1,655 novel SSRs have been isolated from the SSR-enriched library (311) and BAC-end sequences (1,344), a DArT|array has been developed with >16,000 features and >20,000 ESTs from drought and salinity stress challenged tissues|based on Sanger sequencing. Further, a set of 443,969 sequence tags were generated through FLX-454 sequencing|from a pool of normalized cDNA assembled from developmental stages and abiotic stresses challenged tissues of a|reference chickpea genotype (ICC 4958). Analysis of Sanger as well as FLX-454 sequence data provided 103,215|tentative unique sequences (TUSs). In parallel, transcriptomes of drought and Helicoverpa challenged tissues were|sequenced using Solexa sequencing approach. Over 37 million drought responsive tags were obtained from ICC 4958|and ICC 1882 and their alignment with the TUSs provided 26,083 nucleotide variants (SNPs) between the two|genotypes. Similarly, 81.2 million tags were generated from Helicoverpa challenged tissues of ICCC 37 and ICC 506|genotypes and a total of 65,536 nucleotide variants were identified. In collaboration with University of California-Davis, a|pilot Illumina GoldenGate assay for 768 SNPs has been developed. By using above mentioned resources, an integrated|genetic map with >1534 marker loci has been developed based on an interspecific mapping population (C. arietinum ICC|4958 �� C. reticulatum PI 489777). With an objective of identification of candidate markers associated with drought|tolerance (root traits), the most challenging production constraint, two intraspecific mapping populations (ICC 4958 �� ICC|1882 and ICC 283 �� ICC 8261) have been phenotyped for root traits in two environments and genotyped with >250 SSR|markers. Marker-trait analysis has revealed several QTLs including one major QTL in both mapping populations that|contributed up to 30% phenotypic variation for drought-tolerance component traits. This genomic region containing|several QTLs for drought tolerance is being introgressed into three elite chickpea lines (JG 11, ICC 92318 and KAK 2),|using marker-assisted backcrossing (MABC) approach, to develop superior cultivars with enhanced drought tolerance. It|is therefore anticipated that developed genomic resources at large scale should facilitate genomics-assisted breeding|that would lead to sustainable crop production of chickpea in developing countries. |
546 ## - LANGUAGE NOTE | |
Language note | English |
595 ## - COLLECTION | |
Collection | CIMMYT Staff Publications Collection |
942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
Koha item type | Conference proceedings |
Source of classification or shelving scheme | Dewey Decimal Classification |
Date last seen | Total Checkouts | Full call number | Price effective from | Koha item type | Public note | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Withdrawn status | Home library | Current library | Date acquired |
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07/19/2017 | CIS-6173 | 07/19/2017 | Conference proceedings | http://www.gpgr2.com/ | Not Lost | Dewey Decimal Classification | CIMMYT Staff Publications Collection | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Knowledge Center: John Woolston Library | 07/19/2017 |