Knowledge Center Catalog

Development of EST resources in pearl millet and their use in development and mapping of EST-SSRs in four RIL populations (Record no. 8013)

MARC details
000 -LEADER
fixed length control field 03298nam a22005057a 4500
001 - CONTROL NUMBER
control field G94608
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250710103646.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 121211s ||||f| 0 p|p||0|| |
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) CIS-6163
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Rajaram, V.
Affiliation Plant and Animal Genomes Conference, XVIII; San Diego, CA (USA); 9-13 Jan 2010. Abstracts of oral and poster presentations
245 00 - TITLE STATEMENT
Title Development of EST resources in pearl millet and their use in development and mapping of EST-SSRs in four RIL populations
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Date of publication, distribution, etc. 2010
300 ## - PHYSICAL DESCRIPTION
Extent 1 page
520 ## - SUMMARY, ETC.
Summary, etc. Elite pearl millet inbreds ICMB 841-P3 and 863B-P2 (RIL population parents differing for drought and salinity tolerance, downy mildew and blast resistance, and stover and grain quality) were drought stressed at boot-leaf stage using a standard dry-down protocol. Total RNA extracted separately from stressed root and leaf tissues of each inbred was used for cDNA synthesis. 454-sequencing of cDNAs yielded raw EST sequences that were cleaned and assembled using the PLANTTA pipeline. A total of 112,864 unigenes were obtained from the four cDNA samples. A total of 5,800 SNPs from 2,146 contigs were identified between the two parents. Similarly, 499 sequences containing class I SSRs were identified from the unigenes and from these 236 non-redundant EST-SSR primer pairs were designed. PCR products from 139 amplifying EST-SSR primer pairs and parental DNA of crosses ICMB 841-P3 x 863B-P2, H 77/833-2 x PRLT 2/89-33, 81B-P6 x ICMP 451-P8 and PT 732B-P2 x P1449-2-P1 were screened for polymorphism. >50 new EST-SSR markers were mapped for each mapping population and a consensus map developed. These four EST-SSR skeleton-mapped RIL populations have been phenotyped for grain Fe and Zn concentration, salinity tolerance, canopy conductance, downy mildew reaction, rust reaction, and/or blast reaction, and QTLs with closely flanking EST-SSR markers were detected for each of these traits. This study increases the number of mapped pearl millet SSRs by >40%, filling gaps in the earlier linkage maps, and will facilitate SSR-based marker-assisted foreground selection in parts of the pearl millet genome where this was not previously possible.
536 ## - FUNDING INFORMATION NOTE
Text of note Global Wheat Program
546 ## - LANGUAGE NOTE
Language note English
594 ## - STAFFID
StaffID INT2983
595 ## - COLLECTION
Collection CIMMYT Staff Publications Collection
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Bhasker Raj, A.G.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Blummel, M.,
Relator term coaut.
9 (RLIN) 1443
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Choudhary, S.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Hash, C.T.,
Relator term coaut.
9 (RLIN) 2442
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Kavi Kishor, P.B.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Kholova, J.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Kocova, M.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Kumar, S.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Lakshmi Narasu. M.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Nepolean, T.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Pandurangarao, V.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Rai, R.N.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Sahrawat, K.L.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Senthilvel, S.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Singh, G.,
Relator term coaut.
9 (RLIN) 2030
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Supriya,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Thakur, R.P.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Vadez, V.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Varshney, R.K.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Yadav, R.C.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Velu, G.
Miscellaneous information Global Wheat Program
Field link and sequence number INT2983
9 (RLIN) 880
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Sharma, R.K.
Miscellaneous information Formerly Global Wheat Program
Field link and sequence number INT3065
9 (RLIN) 888
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Conference proceedings
Holdings
Date last seen Total Checkouts Full call number Price effective from Koha item type Lost status Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
07/19/2017   CIS-6163 07/19/2017 Conference proceedings Not Lost     CIMMYT Staff Publications Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 07/19/2017

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