Knowledge Center Catalog

Large scale development of BAC-end associated SSR markers in Pigeonpea [Cajanus cajan (L.) Millspaugh] (Record no. 8010)

MARC details
000 -LEADER
fixed length control field 02500nam a22003497a 4500
001 - CONTROL NUMBER
control field 8010
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20240809161240.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 121211s ||||f| 0 p|p||0|| |
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) CIS-6160
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Dubey, A.
Affiliation Plant and Animal Genomes Conference, XVIII; San Diego, CA (USA); 9-13 Jan 2010. Abstracts of oral and poster presentations
245 00 - TITLE STATEMENT
Title Large scale development of BAC-end associated SSR markers in Pigeonpea [Cajanus cajan (L.) Millspaugh]
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Date of publication, distribution, etc. 2010
300 ## - PHYSICAL DESCRIPTION
Extent 1 page
520 ## - SUMMARY, ETC.
Summary, etc. Low levels of polymorphism and lack of sufficient numbers of microsatellite markers are factors that constrain molecular breeding in pigeonpea. BAC-end sequencing approach was used to obtain a set of BAC-associated SSRs (simple sequence repeats), providing a resource for both genetic and physical map analysis. 87,590 pigeonpea BAC end sequences, representing 56.5 Mb from genome, were surveyed for the presence of microsatellite using MIcroSAtellite (MISA). Among 18,149 pigeonpea microsatellites (1 SSR per 32.1Mbp) di-nucleotide motifs were the most abundant (41%), followed by tri-nucleotide motifs (~10%). Most di- and tri-nucleotide sequences were AT-rich (i.e., TA and TAA). With the goal of increasing genetic marker density, 6,590 oligonucleotide primers pairs were designed, 3,072 primer pairs were synthesized and tested. Amplified products were obtained for 2,946 primer pairs and were used to identify polymorphism in a set of 24 pigeonpea genotypes that are parents of different mapping populations segregating for Fusarium wilt (FW), sterility mosaic (SM) and water logging. Number of polymorphic markers identified ranged from 100-415 across different crosses. Genotyping of these polymorphic markers in the respective mapping populations is underway. Marker genotyping data along with phenotyping data obtained from these mapping populations will facilitate mapping of QTLs/genes for the traits of interest to breeders
546 ## - LANGUAGE NOTE
Language note English
595 ## - COLLECTION
Collection CIMMYT Staff Publications Collection
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Bohra, A.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Cheung, F.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Cook, D.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Gothalwal, R.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Kishor, P.B.K.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Kumar, N.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Penmetsa, R.V.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Saxena, K.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Saxena, R.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Singh, D.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Upadhyaya, H.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Varshney, R.,
Relator term coaut.
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Conference proceedings
Source of classification or shelving scheme Dewey Decimal Classification
Holdings
Date last seen Total Checkouts Full call number Price effective from Koha item type Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
07/19/2017   CIS-6160 07/19/2017 Conference proceedings Not Lost Dewey Decimal Classification     CIMMYT Staff Publications Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 07/19/2017

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