Loop-mediated isothermal amplification assay : (Record no. 68954)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03051nab a22003497a 4500 |
| 001 - CONTROL NUMBER | |
| control field | 68954 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250620155719.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250610s2024 -us|||p|op||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 0011-183X |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 1435-0653 (Online) |
| 024 8# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | https://doi.org/10.1002/csc2.21203 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MX-TxCIM |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Wanjala, B.W. |
| 9 (RLIN) | 39329 |
| 245 10 - TITLE STATEMENT | |
| Title | Loop-mediated isothermal amplification assay : |
| Remainder of title | a novel disease diagnostics tool in sweetpotato seed quality assurance |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | United States of America : |
| Name of publisher, distributor, etc. | John Wiley & Sons, |
| Date of publication, distribution, etc. | 2024. |
| 500 ## - GENERAL NOTE | |
| General note | Peer review |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Sweetpotato is an important food crop in global production. However, sweetoptato viruses pose a threat to sustainable agriculture and cause significant economic loss. More than 30 viruses have been reported to date, with sweet potato feathery mottle virus (SPFMV) and sweet potato chlorotic stunt virus (SPCSV) occurring frequently and in combination, causing sweetpotato virus disease. The detection of viruses directly from sweetpotato is still a challenge, and efforts are being made toward this front. Therefore, rapid detection of viruses is critical for effective control. Current diagnostic tests are not sufficiently sensitive to reliably detect viruses directly from sweetpotato and require expensive laboratory equipment and a high level of skilled experience. Loop-mediated isothermal amplification (LAMP) is sensitive and specific for both DNA/RNA amplification; it is affordable and its characteristics make it potentially suitable for on-site testing. LAMP assays have been developed for several sweetpotato viruses: SPCSV, SPFMV, and sweet potato leaf curl virus (and related sweepoviruses). Laboratory validation showed a 100% diagnostic sensitivity for all three viruses. The LAMP on-site testing results were comparable to those of polymerase chain reaction and reverse transcriptase polymerase chain reaction confirmatory laboratory tests. Interlab validation and packaging into affordable kits will ensure high adoption in sub-Saharan Africa. The LAMP assay can be used for field surveys and monitoring the phytosanitary status of pre-basic seed production in quarantine or certification program. This guarantees pathogen-free planting material enters the seed system. We discuss opportunities for LAMP as a diagnostic assay for the rapid detection of sweetpotato viruses and its adoption as a quality control system for planting materials. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Sweet potatoes |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 2711 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Food crops |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 1994 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Sustainable agriculture |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 2327 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Economic losses |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 17225 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Viroses |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 4323 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Seed production |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 1253 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kreuze, J.F. |
| 9 (RLIN) | 18973 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | McEwan, M.A. |
| 9 (RLIN) | 18467 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Low, J.W. |
| 9 (RLIN) | 39305 |
| 773 0# - HOST ITEM ENTRY | |
| Place, publisher, and date of publication | United States of America : John Wiley & Sons, 2024. |
| Related parts | v. 64, no. 3, p. 1183-1192 |
| Title | Crop Science |
| Record control number | G444244 |
| International Standard Serial Number | 0011-183X |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Article |
| Suppress in OPAC | No |
| Date last seen | Total Checkouts | Price effective from | Koha item type | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Withdrawn status | Home library | Current library | Date acquired |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 06/10/2025 | 06/10/2025 | Article | Not Lost | Dewey Decimal Classification | Reprints Collection | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Knowledge Center: John Woolston Library | 06/10/2025 |