Colorimetric detection of Maize chlorotic mottle virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynapthol blue dye (Record no. 68946)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02641nab a22003377a 4500 |
| 001 - CONTROL NUMBER | |
| control field | 68946 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250620155436.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250609s2016 -uk|||p|op||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 2046-2069 (Online) |
| 024 8# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | https://doi.org/10.1039/C5RA20789D |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MX-TxCIM |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Zhanmin Liu |
| 9 (RLIN) | 39296 |
| 245 10 - TITLE STATEMENT | |
| Title | Colorimetric detection of Maize chlorotic mottle virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynapthol blue dye |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | United Kingdom : |
| Name of publisher, distributor, etc. | The Royal Society of Chemistry, |
| Date of publication, distribution, etc. | 2016. |
| 500 ## - GENERAL NOTE | |
| General note | Peer review |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Maize chlorotic mottle virus causes corn lethal necrosis disease, and can be transmitted via infected maize seeds. It remains a challenge to detect this virus to prevent its introduction, infection and wide transmission in fields. For this purpose, a colorimetric assay for the detection of Maize chlorotic mottle virus was developed, which utilizes RT-LAMP and hydroxynapthol blue dye (HNB). The reaction was performed for amplification in one step in a single tube at the optimum conditions (64 °C for 60 min, 150 μM HNB and 2 mM MgSO4). Samples infected with MCMV developed a characteristic sky blue color after the reaction but those uninfected with MCMV or infected with other plant pathogenic viruses did not. The results of the HNB staining method were reconfirmed through gel electrophoresis of the RT-LAMP products. The sensitivity of this assay was 4.8 pg μl−1 of RNA of Maize chlorotic mottle virus per reaction, which was approximately 10-fold higher sensitivity over a conventional RT-PCR test. The results indicate that this assay is highly species-specific, simple, low-cost, and visual for easy detection of Maize chlorotic mottle virus in plant tissues. Therefore, colorimetric detection of Maize chlorotic mottle virus is a potentially useful tool for middle or small-scale corporations and entry-exit inspection and quarantine bureaus to detect maize seeds or plant tissues infected with Maize chlorotic mottle virus. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Maize chlorotic mottle virus |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 9361 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Isothermal processes |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 18087 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Gel electrophoresis |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 22497 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | RNA |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 5052 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Plant tissues |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 13518 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Infection |
| Miscellaneous information | AGROVOC |
| 9 (RLIN) | 11032 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Xueying Xia |
| 9 (RLIN) | 39297 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Cuiyun Yang |
| 9 (RLIN) | 39298 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Junyi Huang |
| 9 (RLIN) | 39300 |
| 773 0# - HOST ITEM ENTRY | |
| Place, publisher, and date of publication | United Kingdom : The Royal Society of Chemistry, 2016. |
| Related parts | v. 6, p. 73–78 |
| Title | RSC Advances |
| International Standard Serial Number | 2046-2069 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Article |
| Suppress in OPAC | No |
| Date last seen | Total Checkouts | Price effective from | Koha item type | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Withdrawn status | Home library | Current library | Date acquired |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 06/09/2025 | 06/09/2025 | Article | Not Lost | Dewey Decimal Classification | Reprints Collection | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Knowledge Center: John Woolston Library | 06/09/2025 |