A bioluminescence assay to detect nitrification inhibitors released from plant roots: a case study with Brachiaria humidicola (Record no. 68922)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 03344nab a22004097a 4500 |
| 001 - CONTROL NUMBER | |
| control field | 68922 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250620154712.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250606s2006 ne |||p|op||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 0032-079X |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 1573-5036 (Online) |
| 024 8# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | https://doi.org/10.1007/s11104-006-9094-3 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MX-TxCIM |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Guntur Venkata Subbarao |
| 9 (RLIN) | 2828 |
| 245 12 - TITLE STATEMENT | |
| Title | A bioluminescence assay to detect nitrification inhibitors released from plant roots: a case study with Brachiaria humidicola |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Netherlands : |
| Name of publisher, distributor, etc. | Springer, |
| Date of publication, distribution, etc. | 2006. |
| 500 ## - GENERAL NOTE | |
| General note | Peer review |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | A bioluminescence assay using recombinant Nitrosomonas europaea was adopted to detect and quantify natural nitrification inhibitors in plant–soil systems. The recombinant strain of N. europaea produces a distinct two-peak luminescence due to the expression of luxAB genes, introduced from Vibrio harveyi, during nitrification. The bioluminescence produced in this assay is highly correlated with NO-2 production (r2 = 0.94). Using the assay, we were able to detect significant amounts of a nitrification inhibitor produced by the roots of Brachiaria humidicola (Rendle) Schweick. We propose that the inhibitory activity produced/released from plants be termed ‘biological nitrification inhibition’ (BNI) to distinguish it from industrially produced inhibitors. The amount of BNI activity produced by roots was expressed in units defined in terms of the action of a standard inhibitor allylthiourea (AT). The inhibitory effect from 0.22 μM AT in an assay containing 18.9 mM of NH+4 is defined as one AT unit of activity. A substantial amount of BNI activity was released from the roots of B. humidicola (15–25 AT unit g–1 root dry wt day–1). The BNI activity released was a function of the growth stage and N content of the plant. Shoot N levels were positively correlated with the release of BNI activity from roots (r2 = 0.76). The inhibitor/s released from B. humidicola roots suppressed soil nitrification. Additions of 20 units of BNI per gram of soil completely inhibited NO-3 formation in a 55-day study and remained functionally stable in the soil for 50 days. Both the ammonia monooxygenase and the hydroxylaminooxidoreductase enzymatic pathways in Nitrosomonas were effectively blocked by the BNI activity released from B. humidicola roots. The proposed bioluminescence assay can be used to characterize and determine the BNI activity of plant roots, thus it could become a powerful tool in genetically exploiting the BNI trait in crops and pastures. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Bioluminescence |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 39206 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Nitrosomonas europaea |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 17175 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Nitrification inhibitors |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 4939 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Ammonia |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 2028 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Oxidants |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 22508 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Bacteria |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 1017 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Vibrio harveyi |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 39207 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Brachiaria humidicola |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 17173 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Roots |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 1755 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ishikawa, T. |
| 9 (RLIN) | 20493 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ito Osamu |
| 9 (RLIN) | 20786 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kazuhiko Nakahara |
| 9 (RLIN) | 2831 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wang, H.Y. |
| 9 (RLIN) | 20788 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Berry, W. |
| 9 (RLIN) | 20496 |
| 773 0# - HOST ITEM ENTRY | |
| Place, publisher, and date of publication | Netherlands : Springer, 2006. |
| Related parts | v. 288, p. 101–112 |
| Title | Plant Soil |
| Record control number | G444682 |
| International Standard Serial Number | 0032-079X |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Article |
| Suppress in OPAC | No |
| Date last seen | Total Checkouts | Price effective from | Koha item type | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Withdrawn status | Home library | Current library | Date acquired |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 06/06/2025 | 06/06/2025 | Article | Not Lost | Dewey Decimal Classification | Reprints Collection | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Knowledge Center: John Woolston Library | 06/06/2025 |