Transcriptomic analysis to understand the nitrogen stress response mechanism in BNI-enabled wheat (Record no. 68875)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 03363nab|a22004337a|4500 |
| 001 - CONTROL NUMBER | |
| control field | 68875 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20251223151608.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250604s2025 s ||||| |||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 1661-6596 |
| 024 8# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | https://doi.org/10.3390/ijms26104610 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MX-TxCIM |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Mishra, C.N. |
| 9 (RLIN) | 14669 |
| 245 10 - TITLE STATEMENT | |
| Title | Transcriptomic analysis to understand the nitrogen stress response mechanism in BNI-enabled wheat |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Basel (Switzerland) : |
| Name of publisher, distributor, etc. | MDPI, |
| Date of publication, distribution, etc. | 2025. |
| 500 ## - GENERAL NOTE | |
| General note | Open Access |
| 500 ## - GENERAL NOTE | |
| General note | Peer review |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | A comparative transcriptomic analysis was conducted for the nitrogen-efficient (BNI-Munal) and derivative parent Munal wheat genotypes to unravel the gene expression patterns across four nitrogen levels (0%, 50%, 75%, and 100%). Analyzing the genes of BNI-enabled wheat helps us understand how they are expressed differently, which heavily influences BNI activity. Grain yield and 1000-grain weight were higher in BNI Munal than in Munal. All the other traits were similar in performance. Varying nitrogen dosages led to significant differences in gene expression patterns between the two genotypes. Genes related to binding and catalytic activity were prevalent among molecular functions, while genes corresponding to cellular anatomical entities dominated the cellular component category. Differential expression was observed in 371 genes at 0%N, 261 genes at 50%N, 303 genes at 75%N, and 736 genes at 100%N. Five unigenes (three upregulated and two downregulated) were consistently expressed across all nitrogen levels. Further analysis of upregulated unigenes identified links to the NrpA gene (involved in nitrogen regulation), tetratricopeptide repeat-containing protein (PPR), and cytokinin dehydrogenase 2. Analysis of downregulated genes pointed to associations with the Triticum aestivum 3BS-specific BAC library, which encodes the NPF (Nitrate and Peptide Transporter Family) and the TaVRN gene family (closely related to the TaNUE1 gene). The five unigenes and one unigene highlighted in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were validated in Munal and BNI Munal. The results obtained will enhance our understanding about gene expression patterns across different nitrogen levels in BNI wheat and help us breed wheat varieties with the BNI trait for improved NUE. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Text in English |
| 597 ## - CGIAR Initiative | |
| Donor or Funder | Japan International Cooperation Agency (JICA) |
| Program & Accelerators | Breeding for Tomorrow |
| CGSpace handle | https://hdl.handle.net/10568/179272 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Nitrification inhibitors |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 4939 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Transcriptomics |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 30116 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Genes |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 3563 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Wheat |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 1310 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sushma Kumari Pawar |
| 9 (RLIN) | 37653 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sharma, S. |
| 9 (RLIN) | 37652 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Thakur, A. |
| 9 (RLIN) | 39066 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sabhyata |
| 9 (RLIN) | 37654 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mishra, S. |
| 9 (RLIN) | 39067 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Satish Kumar |
| 9 (RLIN) | 7883 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Om Prakash Gupta |
| 9 (RLIN) | 28835 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Joshi, A.K. |
| Miscellaneous information | Global Wheat Program |
| -- | Formerly Borlaug Institute for South Asia |
| Field link and sequence number | INT2917 |
| 9 (RLIN) | 873 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tiwari, R. |
| 9 (RLIN) | 9681 |
| 773 0# - HOST ITEM ENTRY | |
| Title | International Journal of Molecular Sciences |
| Related parts | v. 26, no. 10, art. 4610 |
| Place, publisher, and date of publication | Basel (Switzerland) : MDPI, 2025. |
| International Standard Serial Number | 1661-6596 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS | |
| Link text | Open Access through DSpace |
| Uniform Resource Identifier | https://hdl.handle.net/10883/35709 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Article |
| Suppress in OPAC | No |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Date last seen | Total Checkouts | Price effective from | Koha item type | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Withdrawn status | Home library | Current library | Date acquired |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 06/03/2025 | 06/03/2025 | Article | Not Lost | Dewey Decimal Classification | CIMMYT Staff Publications Collection | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Knowledge Center: John Woolston Library | 06/03/2025 |