MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02023nab a22003257a 4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
68074 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20250930155122.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
241107s1980 -us|||po p||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
0021-8561 |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
1520-5118 (Online) |
| 024 8# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
https://doi.org/10.1021/jf60229a023 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Esen, A. |
| 9 (RLIN) |
37036 |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Estimation of protein quality and quantity in corn (Zea mays L.) by assaying protein in two solubility fractions |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
United States of America : |
| Name of publisher, distributor, etc. |
American Chemical Society, |
| Date of publication, distribution, etc. |
1980. |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer review |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
The protein from maize seeds can be recovered in two solubility fractions. Fraction 1 includes zeins (low quality proteins), extracted with 60% tert-butyl alcohol, and fraction 2, called nonzein (high quality proteins), includes albumins, globulins, glutelins, and others, extracted with a high pH buffer containing an ionic detergent (sodium dodecyl sulfate) and a reducing agent (2-mercaptoethanol). Protein content of the two fractions, determined by a simple Coomassie Blue R-250 dye-binding method, using comparable proteins as standards, gives an estimate of protein quantity of the sample. The zein as percent of total extractable protein or the ratio of zein to nonzein can be used to estimate the protein quality. The method offers promise as a mass-screening tool to identify the maize germ plasm with high protein and/or high quality protein. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Approximation |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
34211 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Protein quality |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1223 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Zea mays |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1314 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Solubilization |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
25172 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Zein |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
16128 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Polyacrylamide gel electrophoresis |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
17755 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Germplasm |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1136 |
| 773 0# - HOST ITEM ENTRY |
|---|
| Place, publisher, and date of publication |
United States of America : American Chemical Society, 1980. |
| Related parts |
v. 28, no. 3, p. 529–532 |
| Title |
Journal of Agricultural and Food Chemistry |
| Record control number |
G444388 |
| International Standard Serial Number |
0021-8561 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Article |
| Suppress in OPAC |
No |