Knowledge Center Catalog

Chromatographic and electrophoretic analysis of zein heterogeneity (Record no. 67882)

MARC details
000 -LEADER
fixed length control field 01986nab a22003377a 4500
001 - CONTROL NUMBER
control field 67882
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250912153340.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 240906s1987 -uk|||p|op||| 00| 0 eng d
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 0733-5210
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 1095-9963 (Online)
024 8# - OTHER STANDARD IDENTIFIER
Standard number or code https://doi.org/10.1016/S0733-5210(87)80009-7
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Landry, J.
9 (RLIN) 36086
245 10 - TITLE STATEMENT
Title Chromatographic and electrophoretic analysis of zein heterogeneity
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. United Kingdom :
Name of publisher, distributor, etc. Academic Press Inc. ;
-- Elsevier,
Date of publication, distribution, etc. 1987.
520 ## - SUMMARY, ETC.
Summary, etc. Zein, purified from maize inbred W64A, was chromatographed on a column of hydroxypropylated Sephadex G-100 equilibrated with 75% ethanol containing 0ยท01 M HCI. The fractions obtained were examined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate or acid-urea before and after reduction of disulfide bonds. Mr 22,000 polypeptides, free of Mr 24,000 polypeptides, were isolated from the proteins that were eluted last and were compared with total zein by two-dimensional electrophoresis consisting of isoelectric focusing in the first dimension and acid-urea gel electrophoresis in the second. The Mr 22,000 fraction consisted of at least nineteen proteins exhibiting microheterogeneity. These data indicate that zein comprises two sets of closely related polypeptides that exist free or as oligomers, mainly dimers.
546 ## - LANGUAGE NOTE
Language note Text in English
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Chromatography
Source of heading or term AGROVOC
9 (RLIN) 9037
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Electrophoresis
Source of heading or term AGROVOC
9 (RLIN) 1094
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Zein
Source of heading or term AGROVOC
9 (RLIN) 16128
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Ethanol
Source of heading or term AGROVOC
9 (RLIN) 14591
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Polyacrylamide gel electrophoresis
Source of heading or term AGROVOC
9 (RLIN) 17755
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Urea
Source of heading or term AGROVOC
9 (RLIN) 9626
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Polypeptides
Source of heading or term AGROVOC
9 (RLIN) 36355
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Paulis, J.W.
9 (RLIN) 33897
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Wall, J.S.
9 (RLIN) 33895
773 0# - HOST ITEM ENTRY
Place, publisher, and date of publication United Kingdom : Academic Press Inc. ; Elsevier, 1987.
Related parts v. 5, no. 1, p. 51-60
Title Journal of Cereal Science
Record control number G444514
International Standard Serial Number 0733-5210
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Article
Suppress in OPAC No
Holdings
Date last seen Total Checkouts Price effective from Koha item type Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
09/06/2024   09/06/2024 Article Not Lost Dewey Decimal Classification     Reprints Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 09/06/2024

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