Knowledge Center Catalog

Efficient arabinoxylan assay for wheat : (Record no. 67473)

MARC details
000 -LEADER
fixed length control field 03111nab|a22004217a|4500
001 - CONTROL NUMBER
control field 67473
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20241126084153.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 20245s2024||||mx |||p|op||||00||0|eng|d
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 0733-5210
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 1095-9963 (Online)
024 8# - OTHER STANDARD IDENTIFIER
Standard number or code https://doi.org/10.1016/j.jcs.2024.103897
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Hernandez Espinosa, N.
9 (RLIN) 2182
245 10 - TITLE STATEMENT
Title Efficient arabinoxylan assay for wheat :
Remainder of title Exploring variability and molecular marker associations in Wholemeal and refined flour
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher, distributor, etc. Elsevier Ltd.,
Date of publication, distribution, etc. 2024.
Place of publication, distribution, etc. United States of America :
500 ## - GENERAL NOTE
General note Peer review
500 ## - GENERAL NOTE
General note Open Access
520 ## - SUMMARY, ETC.
Summary, etc. In this study, we present a modified high throughput phloroglucinol colorimetric assay for the quantification of arabinoxylans (AX) in wheat named PentoQuant. The method was downscaled from a 10 ml glass tube to 2 ml microcentrifuge tube format, resulting in a fivefold increase in throughput while concurrently reducing the overall cost and manual labor required for the analysis. Comparison with established colorimetric assays and gas chromatography validates the modified protocol, demonstrating its superior repeatability, rapidity, and simplicity. The effectiveness of the protocol was tested on 606 unique whole meal (WM) and refined flour (RF) bread wheat samples which revealed the presence of more than a twofold variation in both the soluble (WE-AX) and total (TOT-AX) AX fractions in WM (TOT-AX = 31.9–76.1 mg/g; WE-AX = 4.4–12.6 mg/g) and RF (TOT-AX = 7.7–22.4 mg/g; WE-AX = 3.9–11.4 mg/g). Results obtained from the AX quantification were used to test the effectiveness of four molecular markers associated with AX variation and targeting two major genomic regions on the 1BL and 6BS chromosomes. These markers appeared to be particularly relevant for the WE-AX fraction, providing insights to enable marker-assisted breeding.
546 ## - LANGUAGE NOTE
Language note Text in English
591 ## - CATALOGING NOTES
Affiliation Hernandez Espinosa, N. : Not in IRS staff list but CIMMYT Affiliation
591 ## - CATALOGING NOTES
Affiliation Posadas Romano, G. : Not in IRS staff list but CIMMYT Affiliation
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Xylans
Source of heading or term AGROVOC
9 (RLIN) 11597
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Dietary fibres
Source of heading or term AGROVOC
9 (RLIN) 15068
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Genetic markers
Source of heading or term AGROVOC
9 (RLIN) 1848
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Wheat
Source of heading or term AGROVOC
9 (RLIN) 1310
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Posadas Romano, G.
9 (RLIN) 2181
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Dreisigacker, S.
Field link and sequence number INT2692
9 (RLIN) 851
Miscellaneous information Global Wheat Program
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Crossa, J.
Miscellaneous information Genetic Resources Program
Field link and sequence number CCJL01
9 (RLIN) 59
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Crespo-Herrera, L.A.
Field link and sequence number I1706538
9 (RLIN) 2608
Miscellaneous information Global Wheat Program
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Ibba, M.I.
Field link and sequence number 001711897
9 (RLIN) 5836
Miscellaneous information Global Wheat Program
773 0# - HOST ITEM ENTRY
Title Journal of Cereal Science
Related parts v. 117, art. 103897
Place, publisher, and date of publication United States of America : Elsevier Ltd., 2024.
International Standard Serial Number 0733-5210
Record control number G444514
856 4# - ELECTRONIC LOCATION AND ACCESS
Link text Open Access through DSpace
Uniform Resource Identifier https://hdl.handle.net/10883/23164
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Suppress in OPAC No
Source of classification or shelving scheme Dewey Decimal Classification
Holdings
Date last seen Total Checkouts Price effective from Koha item type Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
05/03/2024   05/03/2024 Article Not Lost Dewey Decimal Classification     CIMMYT Staff Publications Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 05/03/2024

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