MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03619nab|a22004697a|4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
66358 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20241108164545.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
20236s2023||||mx |||p|op||||00||0|eng|d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
2223-7747 |
| 024 8# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
https://doi.org/10.3390/plants12122314 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Lopez, L.A. |
| 9 (RLIN) |
4643 |
| 245 1# - TITLE STATEMENT |
|---|
| Title |
Effectiveness of R1-nj anthocyanin marker in the identification of in vivo induced maize haploid embryos |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Name of publisher, distributor, etc. |
MDPI, |
| Date of publication, distribution, etc. |
2023. |
| Place of publication, distribution, etc. |
Basel (Switzerland) : |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer review |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Doubled haploid (DH) technology has become integral to maize breeding programs to expedite inbred line development and increase the efficiency of breeding operations. Unlike many other plant species that use in vitro methods, DH production in maize uses a relatively simple and efficient in vivo haploid induction method. However, it takes two complete crop cycles for DH line generation, one for haploid induction and the other one for chromosome doubling and seed production. Rescuing in vivo induced haploid embryos has the potential to reduce the time for DH line development and improve the efficiency of DH line production. However, the identification of a few haploid embryos (~10%) resulting from an induction cross from the rest of the diploid embryos is a challenge. In this study, we demonstrated that an anthocyanin marker, namely R1-nj, which is integrated into most haploid inducers, can aid in distinguishing haploid and diploid embryos. Further, we tested conditions that enhance R1-nj anthocyanin marker expression in embryos and found that light and sucrose enhance anthocyanin expression, while phosphorous deprivation in the media had no affect. Validating the use of the R1-nj marker for haploid and diploid embryo identification using a gold standard classification based on visual differences among haploids and diploids for characteristics such as seedling vigor, erectness of leaves, tassel fertility, etc., indicated that the R1-nj marker could lead to significantly high false positives, necessitating the use of additional markers for increased accuracy and reliability of haploid embryo identification. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 591 ## - CATALOGING NOTES |
|---|
| Affiliation |
Lopez, L.A. : Not in IRS staff list but CIMMYT Affiliation |
| 591 ## - CATALOGING NOTES |
|---|
| Affiliation |
Ochieng, J. : Not in IRS staff list but CIMMYT Affiliation |
| 591 ## - CATALOGING NOTES |
|---|
| Affiliation |
Pacheco, M. : Not in IRS staff list but CIMMYT Affiliation |
| 591 ## - CATALOGING NOTES |
|---|
| Affiliation |
Martinez, L. : Not in IRS staff list but CIMMYT Affiliation |
| 591 ## - CATALOGING NOTES |
|---|
| Affiliation |
Hamilton Amoshe Omar : Not in IRS staff list but CIMMYT Affiliation |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Doubled haploids |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
29363 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Maize |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1173 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Breeding programmes |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
21704 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Inbred lines |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1155 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Crops |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1069 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ochieng, J. |
| 9 (RLIN) |
31316 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Pacheco, M. |
| 9 (RLIN) |
22013 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Martinez, L. |
| 9 (RLIN) |
3372 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Hamilton Amoshe Omar |
| 9 (RLIN) |
12123 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Gowda, M. |
| Field link and sequence number |
I1705963 |
| 9 (RLIN) |
795 |
| Miscellaneous information |
Global Maize Program |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Prasanna, B.M. |
| Field link and sequence number |
INT3057 |
| 9 (RLIN) |
887 |
| Miscellaneous information |
Global Maize Program |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Field link and sequence number |
I1706696 |
| Personal name |
Dhugga, K. |
| Miscellaneous information |
Genetic Resource Program |
| 9 (RLIN) |
4340 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Chaikam, V. |
| Field link and sequence number |
INT3356 |
| 9 (RLIN) |
936 |
| Miscellaneous information |
Global Maize Program |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Plants |
| Related parts |
v. 12, no. 12, art. 2314 |
| Place, publisher, and date of publication |
Basel (Switzerland) : MDPI, 2023 |
| International Standard Serial Number |
2223-7747 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
|---|
| Link text |
Open Access through DSpace |
| Uniform Resource Identifier |
https://hdl.handle.net/10883/22645 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Suppress in OPAC |
No |
| Source of classification or shelving scheme |
Dewey Decimal Classification |