MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03294nab|a22003977a|4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
65837 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20230818155620.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
20225s2022||||mx |||p|op||||00||0|eng|d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
1664-8021 (Online) |
| 024 8# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
https://doi.org/10.3389/fgene.2022.859676 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Saxesena, R.R. |
| 9 (RLIN) |
29519 |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
SNP discovery using BSR-Seq approach for spot blotch resistance in wheat (Triticum aestivum L.), an essential crop for food security |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Name of publisher, distributor, etc. |
Frontiers, |
| Date of publication, distribution, etc. |
2022. |
| Place of publication, distribution, etc. |
Switzerland : |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer review |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Open Access |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
The pathogenic fungus, Bipolaris sorokiniana, that causes spot blotch (SB) disease of wheat, is a major production constraint in the Eastern Gangetic Plains of South Asia and other warm, humid regions of the world. A recombinant inbred line population was developed and phenotyped at three SB-prone locations in India. The single nucleotide polymorphism (SNP) for SB resistance was identified using a bulked segregant RNA-Seq-based approach, referred to as “BSR-Seq.” Transcriptome sequencing of the resistant parent (YS#24), the susceptible parent (YS#58), and their resistant and susceptible bulks yielded a total of 429.67 million raw reads. The bulk frequency ratio (BFR) of SNPs between the resistant and susceptible bulks was estimated, and selection of SNPs linked to resistance was done using sixfold enrichments in the corresponding bulks (BFR >6). With additional filtering criteria, the number of transcripts was further reduced to 506 with 1055 putative polymorphic SNPs distributed on 21 chromosomes of wheat. Based on SNP enrichment on chromosomal loci, five transcripts were found to be associated with SB resistance. Among the five SB resistance-associated transcripts, four were distributed on the 5B chromosome with putative 52 SNPs, whereas one transcript with eight SNPs was present on chromosome 3B. The SNPs linked to the trait were exposed to a tetra-primer ARMS-PCR assay, and an SNP-based allele-specific marker was identified for SB resistance. The in silico study of these five transcripts showed homology with pathogenesis-related genes; the metabolic pathway also exhibits similar results, suggesting their role in the plant defense mechanism. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Wheat |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1310 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Food security |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1118 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Single nucleotide polymorphisms |
| Miscellaneous information |
AGROVOC |
| 9 (RLIN) |
10805 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Disease resistance |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1077 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Vinod Kumar Mishra |
| 9 (RLIN) |
1734 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ramesh Chand |
| 9 (RLIN) |
1735 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Kumar, U. |
| Miscellaneous information |
Formerly Borlaug Institute for South Asia (BISA) |
| Field link and sequence number |
INT3331 |
| 9 (RLIN) |
921 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Chowdhury, A.K. |
| 9 (RLIN) |
2698 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Bhati, J. |
| 9 (RLIN) |
29520 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Neeraj Budhlakoti |
| 9 (RLIN) |
8360 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Joshi, A.K. |
| 9 (RLIN) |
873 |
| Field link and sequence number |
INT2917 |
| Miscellaneous information |
Global Wheat Program |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Frontiers in Genetics |
| Related parts |
v. 13, art. 859676 |
| Place, publisher, and date of publication |
Switzerland : Frontiers, 2022. |
| International Standard Serial Number |
1664-8021 |
| Record control number |
58093 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
|---|
| Link text |
Open Access through DSpace |
| Uniform Resource Identifier |
https://hdl.handle.net/10883/22370 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Suppress in OPAC |
No |
| Source of classification or shelving scheme |
Dewey Decimal Classification |