MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03128nab|a22003857a|4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
64954 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20231017232351.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
20221s22022|||msz ||ppopp|||00||0|eengdd |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
1664-462X (Online) |
| 024 8# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
https://doi.org/10.3389/fpls.2021.782960 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Field link and sequence number |
001712108 |
| Personal name |
Biswal, A.K. |
| Miscellaneous information |
Formerly Genetic Resources Program |
| 9 (RLIN) |
18209 |
| 245 1# - TITLE STATEMENT |
|---|
| Title |
Novel mutant alleles reveal a role of the extra-large G protein in rice grain filling, panicle architecture, plant growth, and disease resistance |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Name of publisher, distributor, etc. |
Frontiers, |
| Date of publication, distribution, etc. |
2022. |
| Place of publication, distribution, etc. |
Switzerland : |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer review |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Open Access |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Plant growth and grain filling are the key agronomical traits for grain weight and yield of rice. The continuous improvement in rice yield is required for a future sustainable global economy and food security. The heterotrimeric G protein complex containing a canonical α subunit (RGA1) couples extracellular signals perceived by receptors to modulate cell function including plant development and grain weight. We hypothesized that, besides RGA1, three atypical, extra-large GTP-binding protein (XLG) subunits also regulate panicle architecture, plant growth, development, grain weight, and disease resistance. Here, we identified a role of XLGs in agronomic traits and stress tolerance by genetically ablating all three rice XLGs individually and in combination using the CRISPR/Cas9 genome editing in rice. For this study, eight (three single, two double, and three triple) null mutants were selected. Three XLG proteins combinatorically regulate seed filling, because loss confers a decrease in grain weight from 14% with loss of one XLG and loss of three to 32% decrease in grain weight. Null mutations in XLG2 and XLG4 increase grain size. The mutants showed significantly reduced panicle length and number per plant including lesser number of grains per panicle compared to the controls. Loss-of-function of all individual XLGs contributed to 9% more aerial biomass compared to wild type (WT). The double mutant showed improved salinity tolerance. Moreover, loss of the XLG gene family confers hypersensitivity to pathogens. Our findings suggest that the non-canonical XLGs play important roles in regulating rice plant growth, grain filling, panicle phenotype, stress tolerance, and disease resistance. Genetic manipulation of XLGs has the potential to improve agronomic properties in rice. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
CRISPR |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
26508 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Rice |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1243 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Proteins |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1224 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Plant growth |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
21209 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Disease resistance |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1077 |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Wu, T.-Y. |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Urano, D. |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Pelissier, R. |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Morel, J.-B. |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Jones, A.M. |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Biswal, A.K. |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Frontiers in Plant Science |
| Related parts |
v. 12, art. 782960 |
| Place, publisher, and date of publication |
Switzerland : Frontiers, 2022 |
| Record control number |
56875 |
| International Standard Serial Number |
1664-462X |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
|---|
| Link text |
Open Access through DSpace |
| Uniform Resource Identifier |
https://hdl.handle.net/10883/21911 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Suppress in OPAC |
No |
| Source of classification or shelving scheme |
Dewey Decimal Classification |