Comparative proteomic and physiological analyses of two divergent maize inbred lines provide more insights into drought-stress tolerance mechanisms (Record no. 64731)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03646nab a22003737a 4500 |
| 001 - CONTROL NUMBER | |
| control field | 64731 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20211217230304.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200115s2018 sz |||p|op||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 1422-0067 (Online) |
| 024 8# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | https://doi.org/10.3390/ijms19103225 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MX-TxCIM |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 26056 |
| Personal name | Zenda, T. |
| 245 10 - TITLE STATEMENT | |
| Title | Comparative proteomic and physiological analyses of two divergent maize inbred lines provide more insights into drought-stress tolerance mechanisms |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Basel (Switzerland) : |
| Name of publisher, distributor, etc. | MDPI, |
| Date of publication, distribution, etc. | 2018. |
| 500 ## - GENERAL NOTE | |
| General note | Peer review |
| 500 ## - GENERAL NOTE | |
| General note | Open Access |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Drought stress is the major abiotic factor threatening maize (Zea mays L.) yield globally. Therefore, revealing the molecular mechanisms fundamental to drought tolerance in maize becomes imperative. Herein, we conducted a comprehensive comparative analysis of two maize inbred lines contrasting in drought stress tolerance based on their physiological and proteomic responses at the seedling stage. Our observations showed that divergent stress tolerance mechanisms exist between the two inbred-lines at physiological and proteomic levels, with YE8112 being comparatively more tolerant than MO17 owing to its maintenance of higher relative leaf water and proline contents, greater increase in peroxidase (POD) activity, along with decreased level of lipid peroxidation under stressed conditions. Using an iTRAQ (isobaric tags for relative and absolute quantification)-based method, we identified a total of 721 differentially abundant proteins (DAPs). Amongst these, we fished out five essential sets of drought responsive DAPs, including 13 DAPs specific to YE8112, 107 specific DAPs shared between drought-sensitive and drought-tolerant lines after drought treatment (SD_TD), three DAPs of YE8112 also regulated in SD_TD, 84 DAPs unique to MO17, and five overlapping DAPs between the two inbred lines. The most significantly enriched DAPs in YE8112 were associated with the photosynthesis antenna proteins pathway, whilst those in MO17 were related to C5-branched dibasic acid metabolism and RNA transport pathways. The changes in protein abundance were consistent with the observed physiological characterizations of the two inbred lines. Further, quantitative real-time polymerase chain reaction (qRT-PCR) analysis results confirmed the iTRAQ sequencing data. The higher drought tolerance of YE8112 was attributed to: activation of photosynthesis proteins involved in balancing light capture and utilization; enhanced lipid-metabolism; development of abiotic and biotic cross-tolerance mechanisms; increased cellular detoxification capacity; activation of chaperones that stabilize other proteins against drought-induced denaturation; and reduced synthesis of redundant proteins to help save energy to battle drought stress. These findings provide further insights into the molecular signatures underpinning maize drought stress tolerance. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 18275 |
| Topical term or geographic name as entry element | Proteomics |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 1224 |
| Topical term or geographic name as entry element | Proteins |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 1081 |
| Topical term or geographic name as entry element | Drought stress |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 26057 |
| Topical term or geographic name as entry element | Physiological response |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 1314 |
| Topical term or geographic name as entry element | Zea mays |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 26058 |
| Personal name | Songtao Liu |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 26059 |
| Personal name | Xuan Wang |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 26060 |
| Personal name | Hongyu Jin |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 26061 |
| Personal name | Guo Liu |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 26062 |
| Personal name | Huijun Duan |
| 773 0# - HOST ITEM ENTRY | |
| Place, publisher, and date of publication | Basel (Switzerland) : MDPI, 2018. |
| Related parts | v. 19, no. 10, art. 3225 |
| Title | International Journal of Molecular Sciences |
| Record control number | 57216 |
| International Standard Serial Number | 1422-0067 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS | |
| Link text | Click here to access online |
| Uniform Resource Identifier | https://doi.org/10.3390/ijms19103225 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Article |
| Suppress in OPAC | No |
| Date last seen | Total Checkouts | Price effective from | Koha item type | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Withdrawn status | Home library | Current library | Date acquired |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 12/15/2021 | 12/15/2021 | Article | Not Lost | Dewey Decimal Classification | Reprints Collection | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Knowledge Center: John Woolston Library | 12/15/2021 |