Effects of grain processing methods on the expression of genes involved in volatile fatty acid transport and pH regulation, and keratinization in rumen epithelium of beef cattle (Record no. 64713)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03499nab|a22004697a|4500 |
| 001 - CONTROL NUMBER | |
| control field | 64713 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20211217225813.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 211206s2018 xxu||||| |||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 1932-6203 (Online) |
| 024 8# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | https://doi.org/10.1371/journal.pone.0198963 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MX-TxCIM |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | del Bianco Benedeti, P. |
| 9 (RLIN) | 25962 |
| 245 10 - TITLE STATEMENT | |
| Title | Effects of grain processing methods on the expression of genes involved in volatile fatty acid transport and pH regulation, and keratinization in rumen epithelium of beef cattle |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | San Francisco, CA (USA) : |
| Name of publisher, distributor, etc. | Public Library of Science |
| Date of publication, distribution, etc. | 2018. |
| 500 ## - GENERAL NOTE | |
| General note | Peer review |
| 500 ## - GENERAL NOTE | |
| General note | Open Access |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Two experiments were carried out to evaluate the effects of corn and sorghum with different processing methods on the expression of genes involved in volatile fatty acids transport and pH regulation, and ruminal keratinization in rumen epithelium of finishing bulls. For Exp. 1, five rumen cannulated Nellore bulls were used in a 5x5 Latin square arrangement, with 14 d for adaptation and 9 d for sample collection. Treatments were: dry ground corn, dry ground sorghum, reconstituted corn, reconstituted sorghum, and control (forage-based diet). Samples of rumen epithelium from ventral sac were excised, rinsed, snap-frozen and stored at -80°C until total RNA isolation and quantitative real-time PCR analysis. In the Exp. 2, 24 Nellore bulls were assigned to a completely randomized design lasting 168 d. Experimental treatments were similar to those at Exp. 1, but without the control treatment. After the experimental period, bulls were slaughtered and rumen epithelium samples were rapidly excised for further histological analysis. Rumen epithelial tissue from animals fed reconstituted corn had lower expression of downregulated-in-adenoma (P = 0.03) and Na+/H+ exchanger 2 (trend; P = 0.09). The expression of Na+/ H+ exchanger 1 (P = 0.10) and putative anion transporter (P = 0.06) tended to be lower in rumen epithelium of bulls fed reconstituted grains. Ruminal concentration of valerate was greater for animals fed reconstituted grain (P = 0.01). Likewise, animals fed reconstituted corn tended to have greater butyrate ruminal concentration (P = 0.08). Keratinized layer thickness did not differ among treatments (P > 0.10). Therefore, reconstituted grains (especially corn) decrease the mRNA expression of genes involved in volatile fatty acids transport and pH control in the rumen epithelium. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Epithelium |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 25963 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Maize |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 1173 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Sorghum |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 2002 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Diet |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 5374 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Gene Expression |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 8835 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Fatty acids |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 12373 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Fermentation |
| 9 (RLIN) | 12531 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Anions |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 25964 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | de Castro Silva, B. |
| 9 (RLIN) | 25965 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Pacheco, M.V.C. |
| 9 (RLIN) | 25966 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Serão, N.V.L. |
| 9 (RLIN) | 25967 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Filho, I.C. |
| 9 (RLIN) | 25968 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lopes, M.M. |
| 9 (RLIN) | 25969 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Marcondes, M.I. |
| 9 (RLIN) | 25970 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mantovani, H.C. |
| 9 (RLIN) | 25971 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Valadares Filho, S.C. |
| 9 (RLIN) | 25972 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Detmann, E. |
| 9 (RLIN) | 25973 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Duarte, M.S. |
| 9 (RLIN) | 25974 |
| 773 0# - HOST ITEM ENTRY | |
| Title | PLoS ONE |
| Related parts | v. 13, no. 6, e0198963 |
| Place, publisher, and date of publication | San Francisco, CA (USA) : Public Library of Science, 2018. |
| International Standard Serial Number | 1932-6203 |
| Record control number | G94957 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS | |
| Link text | Click here to access online |
| Uniform Resource Identifier | https://doi.org/10.1371/journal.pone.0198963 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Article |
| Suppress in OPAC | No |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Date last seen | Total Checkouts | Price effective from | Koha item type | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Withdrawn status | Home library | Current library | Date acquired |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 12/13/2021 | 12/13/2021 | Article | Not Lost | Dewey Decimal Classification | Reprints Collection | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Knowledge Center: John Woolston Library | 12/13/2021 |