MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02919nab|a22003497a|4500 |
| 001 - CONTROL NUMBER |
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| control field |
64703 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20211213160603.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
202102s2019||||sz |||p|op||||00||0|eng|d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
1664-8021 (Online) |
| 024 8# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
https://doi.org/10.3389/fgene.2019.00253 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Liangzhen Zhao |
| 9 (RLIN) |
25915 |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Analysis of transcriptome and epitranscriptome in plants using PacBio Iso-Seq and nanopore-based direct RNA sequencing |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
Switzerland : |
| Name of publisher, distributor, etc. |
Frontiers, |
| Date of publication, distribution, etc. |
2019. |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer review |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Open Access |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Nanopore sequencing from Oxford Nanopore Technologies (ONT) and Pacific BioSciences (PacBio) single-molecule real-time (SMRT) long-read isoform sequencing (Iso-Seq) are revolutionizing the way transcriptomes are analyzed. These methods offer many advantages over most widely used high-throughput short-read RNA sequencing (RNA-Seq) approaches and allow a comprehensive analysis of transcriptomes in identifying full-length splice isoforms and several other post-transcriptional events. In addition, direct RNA-Seq provides valuable information about RNA modifications, which are lost during the PCR amplification step in other methods. Here, we present a comprehensive summary of important applications of these technologies in plants, including identification of complex alternative splicing (AS), full-length splice variants, fusion transcripts, and alternative polyadenylation (APA) events. Furthermore, we discuss the impact of the newly developed nanopore direct RNA-Seq in advancing epitranscriptome research in plants. Additionally, we summarize computational tools for identifying and quantifying full-length isoforms and other co/post-transcriptional events and discussed some of the limitations with these methods. Sequencing of transcriptomes using these new single-molecule long-read methods will unravel many aspects of transcriptome complexity in unprecedented ways as compared to previous short-read sequencing approaches. Analysis of plant transcriptomes with these new powerful methods that require minimum sample processing is likely to become the norm and is expected to uncover novel co/post-transcriptional gene regulatory mechanisms that control biological outcomes during plant development and in response to various stresses. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
RNA |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
5052 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Transcriptome |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
20139 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Plant Genetics |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
9025 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Hangxiao Zhang |
| 9 (RLIN) |
25916 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Kohnen, M.V. |
| 9 (RLIN) |
25917 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Prasad, K.V.S.V. |
| 9 (RLIN) |
8019 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Lianfeng Gu |
| 9 (RLIN) |
25918 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Reddy, A.S.N. |
| 9 (RLIN) |
25919 |
| 773 0# - HOST ITEM ENTRY |
|---|
| Related parts |
v. 10, art. 253 |
| Place, publisher, and date of publication |
Switzerland : Frontiers, 2019. |
| International Standard Serial Number |
1664-8021 |
| Title |
Frontiers in Genetics |
| Record control number |
58093 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
|---|
| Link text |
Click here to access online |
| Uniform Resource Identifier |
https://doi.org/10.3389/fgene.2019.00253 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Suppress in OPAC |
No |
| Source of classification or shelving scheme |
Dewey Decimal Classification |