Assessment of corn starch as substitute for agarose in DNA gel electrophoresis (Record no. 64136)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02620nab|a22004217a|4500 |
| 001 - CONTROL NUMBER | |
| control field | 64136 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20211006085231.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200910s2021||||xxk|||p|op||||00||0|eng|d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 1756-0500 |
| 024 8# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | https://doi.org/10.1186/s13104-021-05483-1 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MX-TxCIM |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Djankpa, F.T. |
| 9 (RLIN) | 22496 |
| 245 10 - TITLE STATEMENT | |
| Title | Assessment of corn starch as substitute for agarose in DNA gel electrophoresis |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | London (United Kingdom) : |
| Name of publisher, distributor, etc. | BioMed Central, |
| Date of publication, distribution, etc. | 2021. |
| 500 ## - GENERAL NOTE | |
| General note | Peer review |
| 500 ## - GENERAL NOTE | |
| General note | Open Access |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Objective: The use of agarose in nucleic acid electrophoresis is the gold standard. However, agarose is very expensive and not readily available in resource limited developing countries like Ghana. Hence, finding a more affordable and readily available alternative to agarose will be a major boost to molecular research in developing countries. This study was aimed at investigating the use of corn starch as a potential substitute for agarose in DNA gel electrophoresis. Results: Genomic deoxyribonucleic acid (DNA) extracted from Plasmodium falciparum and primers were obtained from the West African Centre for Cell Biology of Infectious Pathogens and amplified using polymerase chain reaction. The amplicon was run on agarose gel to ascertain the molecular weight (as a positive control). When visualized under both blue light and ultraviolet light, the DNA and ladder showed clear and clean bands with the expected molecular weight. Corn starch was then modified with sodium borate buffer, casted into a gel and used to run the same DNA sample. Our findings indicated that similar to agarose, the DNA sample and ladder migrated successfully through the modified starch gel but no bands were visible when visualized under blue and ultra-violet light. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 22497 |
| Topical term or geographic name as entry element | Gel electrophoresis |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 13434 |
| Topical term or geographic name as entry element | DNA |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Maize |
| Miscellaneous information | AGROVOC |
| Source of heading or term | |
| 9 (RLIN) | 1173 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 5810 |
| Topical term or geographic name as entry element | Starch |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 22498 |
| Topical term or geographic name as entry element | Agarose |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22499 |
| Personal name | Wiafe, G.A. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22500 |
| Personal name | Boateng, B.N. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22501 |
| Personal name | Tsegah, K.M. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22502 |
| Personal name | Essien-Baidoo, S. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22503 |
| Personal name | Ulanja, M.B. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22504 |
| Personal name | Affram, K.O. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22505 |
| Personal name | Ussif, A.M. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22506 |
| Personal name | Agyeman, D.O. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22507 |
| Personal name | Asante, G. |
| 773 0# - HOST ITEM ENTRY | |
| Title | BMC Research Notes |
| Related parts | v. 14, art. 73 |
| Place, publisher, and date of publication | London (United Kingdom) : BioMed Central, 2021. |
| International Standard Serial Number | 1756-0500 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS | |
| Link text | Click here to access online |
| Uniform Resource Identifier | https://doi.org/10.1186/s13104-021-05483-1 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Article |
| Suppress in OPAC | No |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Date last seen | Total Checkouts | Price effective from | Koha item type | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Withdrawn status | Home library | Current library | Date acquired |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 08/27/2021 | 08/27/2021 | Article | Not Lost | Dewey Decimal Classification | Reprints Collection | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Knowledge Center: John Woolston Library | 08/27/2021 |