Insights into the genome and secretome of Fusarium metavorans DSM105788 by cultivation on agro-residual biomass and synthetic nutrient sources (Record no. 64131)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03463nab a22004577a 4500 |
| 001 - CONTROL NUMBER | |
| control field | 64131 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20211006074802.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210625s2021 xxk|||p|op||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 1754-6834 |
| 024 8# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | https://doi.org/10.1186/s13068-021-01927-9 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MX-TxCIM |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22463 |
| Personal name | Brandt, S.C. |
| 245 10 - TITLE STATEMENT | |
| Title | Insights into the genome and secretome of Fusarium metavorans DSM105788 by cultivation on agro-residual biomass and synthetic nutrient sources |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | London (United Kingdom) : |
| Name of publisher, distributor, etc. | BioMed Central, |
| Date of publication, distribution, etc. | 2021. |
| 500 ## - GENERAL NOTE | |
| General note | Peer review |
| 500 ## - GENERAL NOTE | |
| General note | Open Access |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Background: The transition to a biobased economy involving the depolymerization and fermentation of renewable agro-industrial sources is a challenge that can only be met by achieving the efficient hydrolysis of biomass to monosaccharides. In nature, lignocellulosic biomass is mainly decomposed by fungi. We recently identified six efficient cellulose degraders by screening fungi from Vietnam. Results: We characterized a high-performance cellulase-producing strain, with an activity of 0.06 U/mg, which was identified as a member of the Fusarium solani species complex linkage 6 (Fusarium metavorans), isolated from mangrove wood (FW16.1, deposited as DSM105788). The genome, representing nine potential chromosomes, was sequenced using PacBio and Illumina technology. In-depth secretome analysis using six different synthetic and artificial cellulose substrates and two agro-industrial waste products identified 500 proteins, including 135 enzymes assigned to five different carbohydrate-active enzyme (CAZyme) classes. The F. metavorans enzyme cocktail was tested for saccharification activity on pre-treated sugarcane bagasse, as well as untreated sugarcane bagasse and maize leaves, where it was complemented with the commercial enzyme mixture Accellerase 1500. In the untreated sugarcane bagasse and maize leaves, initial cell wall degradation was observed in the presence of at least 196 µg/mL of the in-house cocktail. Increasing the dose to 336 µg/mL facilitated the saccharification of untreated sugarcane biomass, but had no further effect on the pre-treated biomass. Conclusion: Our results show that F. metavorans DSM105788 is a promising alternative pre-treatment for the degradation of agro-industrial lignocellulosic materials. The enzyme cocktail promotes the debranching of biopolymers surrounding the cellulose fibers and releases reduced sugars without process disadvantages or loss of carbohydrates. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Fusarium |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 2705 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Genomes |
| Miscellaneous information | AGROVOC |
| Source of heading or term | |
| 9 (RLIN) | 1131 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Mass spectrometry |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 11789 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Proteomics |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 18275 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Cellulose |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 4395 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Biomass |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 1897 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22464 |
| Personal name | Brognaro, H. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22465 |
| Personal name | Ali, A. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22466 |
| Personal name | Ellinger, B. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22467 |
| Personal name | Maibach, K. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22468 |
| Personal name | Rühl, M. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22469 |
| Personal name | Wrenger, C. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22470 |
| Personal name | Schlüter, H. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22471 |
| Personal name | Schäfer, W. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22472 |
| Personal name | Betzel, C. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22473 |
| Personal name | Janssen, S. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 22474 |
| Personal name | Gand, M. |
| 773 0# - HOST ITEM ENTRY | |
| Title | Biotechnology for Biofuels |
| Related parts | v. 14, art. 74 |
| Place, publisher, and date of publication | London (United Kingdom) : BioMed Central, 2021. |
| International Standard Serial Number | 1754-6834 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | https://doi.org/10.1186/s13068-021-01927-9 |
| Link text | Click here to access online |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Article |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Suppress in OPAC | No |
| Date last seen | Total Checkouts | Price effective from | Koha item type | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Withdrawn status | Home library | Current library | Date acquired |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 08/25/2021 | 08/25/2021 | Article | Not Lost | Dewey Decimal Classification | Reprints Collection | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Knowledge Center: John Woolston Library | 08/25/2021 |