MARC details
| 000 -LEADER |
|---|
| fixed length control field |
00595nab|a22002177a|4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
64129 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20210902154554.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
202112s2021||||xxk|||p|op||||00||0|eng|d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
1474-7596 |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
1474-760X (Online) |
| 024 8# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
https://doi.org/10.1186/s13059-020-02242-6 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Xiaoyu Yang |
| 9 (RLIN) |
22452 |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Widespread occurrence of microRNA-mediated target cleavage on membrane-bound polysomes |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
United Kingdom : |
| Name of publisher, distributor, etc. |
BioMed Central, |
| Date of publication, distribution, etc. |
2021. |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer review |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Open Access |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Background: Small RNAs (sRNAs) including microRNAs (miRNAs) and small interfering RNAs (siRNAs) serve as core players in gene silencing at transcriptional and post-transcriptional levels in plants, but their subcellular localization has not yet been well studied, thus limiting our mechanistic understanding of sRNA action. Results: We investigate the cytoplasmic partitioning of sRNAs and their targets globally in maize (Zea mays, inbred line “B73”) and rice (Oryza sativa, cv. “Nipponbare”) by high-throughput sequencing of polysome-associated sRNAs and 3′ cleavage fragments, and find that both miRNAs and a subset of 21-nucleotide (nt)/22-nt siRNAs are enriched on membrane-bound polysomes (MBPs) relative to total polysomes (TPs) across different tissues. Most of the siRNAs are generated from transposable elements (TEs), and retrotransposons positively contributed to MBP overaccumulation of 22-nt TE-derived siRNAs (TE-siRNAs) as opposed to DNA transposons. Widespread occurrence of miRNA-mediated target cleavage is observed on MBPs, and a large proportion of these cleavage events are MBP-unique. Reproductive 21PHAS (21-nt phasiRNA-generating) and 24PHAS (24-nt phasiRNA-generating) precursors, which were commonly considered as noncoding RNAs, are bound by polysomes, and high-frequency cleavage of 21PHAS precursors by miR2118 and 24PHAS precursors by miR2275 is further detected on MBPs. Reproductive 21-nt phasiRNAs are enriched on MBPs as opposed to TPs, whereas 24-nt phasiRNAs are nearly completely devoid of polysome occupancy. Conclusions: MBP overaccumulation is a conserved pattern for cytoplasmic partitioning of sRNAs, and endoplasmic reticulum (ER)-bound ribosomes function as an independent regulatory layer for miRNA-induced gene silencing and reproductive phasiRNA biosynthesis in maize and rice. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Zea mays |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1314 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Oryza sativa |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
7678 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
microRNA |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
22453 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
RNA |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
5052 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Chenjiang You |
| 9 (RLIN) |
22454 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Xufeng Wang |
| 9 (RLIN) |
22455 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Lei Gao |
| 9 (RLIN) |
14851 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Beixin Mo |
| 9 (RLIN) |
22456 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Lin Liu |
| 9 (RLIN) |
22457 |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Xuemei Chen |
| 9 (RLIN) |
22458 |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Genome Biology |
| Related parts |
v. 22, art. 15 |
| Place, publisher, and date of publication |
United Kingdom : BioMed Central, 2021. |
| International Standard Serial Number |
1474-760X |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
|---|
| Link text |
Click here to access online |
| Uniform Resource Identifier |
https://doi.org/10.1186/s13059-020-02242-6 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Suppress in OPAC |
No |
| Source of classification or shelving scheme |
Dewey Decimal Classification |