MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02562nab|a22003497a|4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
63885 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20211006074801.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
200423s2021||||xxk|||p|op||||00||0|eng|d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
2045-2322 |
| 024 8# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
https://doi.org/10.1038/s41598-021-91098-2 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Sedaghatjoo, S. |
| 9 (RLIN) |
20638 |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Development of a loop-mediated isothermal amplification assay for the detection of Tilletia controversa based on genome comparison |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
London (United Kingdom) : |
| Name of publisher, distributor, etc. |
Nature Publishing Group, |
| Date of publication, distribution, etc. |
2021. |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer review |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Open Access |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Tilletia controversa causing dwarf bunt of wheat is a quarantine pathogen in several countries. Therefore, its specific detection is of great phytosanitary importance. Genomic regions routinely used for phylogenetic inferences lack suitable polymorphisms for the development of species-specific markers. We therefore compared 21 genomes of six Tilletia species to identify DNA regions that were unique and conserved in all T. controversa isolates and had no or limited homology to other Tilletia species. A loop-mediated isothermal amplification (LAMP) assay for T. controversa was developed based on one of these DNA regions. The specificity of the assay was verified using 223 fungal samples comprising 43 fungal species including 11 Tilletia species, in particular 39 specimens of T. controversa, 92 of T. caries and 40 of T. laevis, respectively. The assay specifically amplified genomic DNA of T. controversa from pure cultures and teliospores. Only Tilletia trabutii generated false positive signals. The detection limit of the LAMP assay was 5 pg of genomic DNA per reaction. A test performance study that included five laboratories in Germany resulted in 100% sensitivity and 97.7% specificity of the assay. Genomic regions, specific to common bunt (Tilletia caries and Tilletia laevis together) are also provided. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
20639 |
| Topical term or geographic name as entry element |
Tilletia controversa |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
20640 |
| Topical term or geographic name as entry element |
Detection |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Genomes |
| Miscellaneous information |
AGROVOC |
| Source of heading or term |
|
| 9 (RLIN) |
1131 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Forster, M.K. |
| 9 (RLIN) |
20641 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Niessen, L. |
| 9 (RLIN) |
20642 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Karlovsky, P. |
| 9 (RLIN) |
20643 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Killermann, B. |
| 9 (RLIN) |
20644 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Maier, W. |
| 9 (RLIN) |
190 |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Nature Scientific Reports |
| Related parts |
v. 11, art. 11611 |
| Place, publisher, and date of publication |
London (United Kingdom) : Nature Publishing Group, 2021. |
| International Standard Serial Number |
2045-2322 |
| Record control number |
a58025 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
|---|
| Link text |
Click here to access online |
| Uniform Resource Identifier |
https://doi.org/10.1038/s41598-021-91098-2 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Suppress in OPAC |
No |
| Source of classification or shelving scheme |
Dewey Decimal Classification |