Knowledge Center Catalog

Analysis of methods for quantifying yeast cell concentration in complex lignocellulosic fermentation processes (Record no. 63884)

MARC details
000 -LEADER
fixed length control field 00595nab|a22002177a|4500
001 - CONTROL NUMBER
control field 63884
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20210629230112.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 200423s2021||||xxk|||p|op||||00||0|eng|d
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 2045-2322
024 8# - OTHER STANDARD IDENTIFIER
Standard number or code https://doi.org/10.1038/s41598-021-90703-8
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Ruifei Wang
9 (RLIN) 20632
245 10 - TITLE STATEMENT
Title Analysis of methods for quantifying yeast cell concentration in complex lignocellulosic fermentation processes
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. London (United Kingdom) :
Name of publisher, distributor, etc. Nature Publishing Group,
Date of publication, distribution, etc. 2021.
500 ## - GENERAL NOTE
General note Peer review
500 ## - GENERAL NOTE
General note Open Access
520 ## - SUMMARY, ETC.
Summary, etc. Cell mass and viability are tightly linked to the productivity of fermentation processes. In 2nd generation lignocellulose-based media quantitative measurement of cell concentration is challenging because of particles, auto-fluorescence, and intrinsic colour and turbidity of the media. We systematically evaluated several methods for quantifying total and viable yeast cell concentrations to validate their use in lignocellulosic media. Several automated cell counting systems and stain-based viability tests had very limited applicability in such samples. In contrast, manual cell enumeration in a hemocytometer, plating and enumeration of colony forming units, qPCR, and in situ dielectric spectroscopy were further investigated. Parameter optimization to measurements in synthetic lignocellulosic media, which mimicked typical lignocellulosic fermentation conditions, resulted in statistically significant calibration models with good predictive capacity for these four methods. Manual enumeration of cells in a hemocytometer and of CFU were further validated for quantitative assessment of cell numbers in simultaneous saccharification and fermentation experiments on steam-exploded wheat straw. Furthermore, quantitative correlations could be established between these variables and in situ permittivity. In contrast, qPCR quantification suffered from inconsistent DNA extraction from the lignocellulosic slurries. Development of reliable and validated cell quantification methods and understanding their strengths and limitations in lignocellulosic contexts, will enable further development, optimization, and control of lignocellulose-based fermentation processes.
546 ## - LANGUAGE NOTE
Language note Text in English
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Source of heading or term AGROVOC
9 (RLIN) 12531
Topical term or geographic name as entry element Fermentation
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Source of heading or term AGROVOC
9 (RLIN) 16740
Topical term or geographic name as entry element Yeasts
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Source of heading or term AGROVOC
9 (RLIN) 20633
Topical term or geographic name as entry element Cell counting
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Lorantfy, B.
9 (RLIN) 20634
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Fusco, S.
9 (RLIN) 20635
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Olsson, L.
9 (RLIN) 20636
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Franzén, C.J.
9 (RLIN) 20637
773 0# - HOST ITEM ENTRY
Title Nature Scientific Reports
Related parts v. 11, art. 11293
Place, publisher, and date of publication London (United Kingdom) : Nature Publishing Group, 2021.
International Standard Serial Number 2045-2322
Record control number a58025
856 4# - ELECTRONIC LOCATION AND ACCESS
Link text Click here to access online
Uniform Resource Identifier https://doi.org/10.1038/s41598-021-90703-8
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Suppress in OPAC No
Source of classification or shelving scheme Dewey Decimal Classification
Holdings
Date last seen Total Checkouts Price effective from Koha item type Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
06/28/2021   06/28/2021 Article Not Lost Dewey Decimal Classification     Reprints Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 06/28/2021

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