MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03731nab|a22004577a|4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
63845 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20230711184627.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
200910s2021||||sz |||p|op||||00||0|eng|d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
1664-462X |
| 024 8# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
https://doi.org/10.3389/fpls.2021.649308 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Awata, L.A.O. |
| 9 (RLIN) |
9174 |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Introgression of maize lethal necrosis resistance quantitative trait loci into susceptible maize populations and validation of the resistance under field conditions in Naivasha, Kenya |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
Switzerland : |
| Name of publisher, distributor, etc. |
Frontiers, |
| Date of publication, distribution, etc. |
2021. |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer review |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Open Access |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Maize lethal necrosis (MLN), resulting from co-infection by maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV) can cause up to 100% yield losses in maize in Africa under serious disease conditions. Maize improvement through conventional backcross (BC) takes many generations but can significantly be shortened when molecular tools are utilized in the breeding process. We used a donor parent (KS23-6) to transfer quantitative trait loci (QTL) for resistance to MLN into nine adapted but MLN susceptible lines. Nurseries were established in Kiboko, Kenya during 2015–2017 seasons and BC3F2 progeny were developed using marker assisted backcrossing (MABC) approach. Six single nucleotide polymorphism (SNP) markers linked to QTL for resistance to MLN were used to genotype 2,400 BC3F2 lines using Kompetitive Allele Specific PCR (KASP) platform. We detected that two of the six QTL had major effects for resistance to MLN under artificial inoculation field conditions in 56 candidate BC3F2 lines. To confirm whether these two QTL are reproducible under different field conditions, the 56 BC3F2 lines including their parents were evaluated in replicated trials for two seasons under artificial MLN inoculations in Naivasha, Kenya in 2018. Strong association of genotype with phenotype was detected. Consequently, 19 superior BC3F2 lines with favorable alleles and showing improved levels of resistance to MLN under artificial field inoculation were identified. These elite lines represent superior genetic resources for improvement of maize hybrids for resistance to MLN. However, 20 BC3F2 lines were fixed for both KASP markers but were susceptible to MLN under field conditions, which could suggest weak linkage between the KASP markers and target genes. The validated two major QTL can be utilized to speed up the breeding process but additional loci need to be identified between the KASP markers and the resistance genes to strengthen the linkage. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Maize |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1173 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
15354 |
| Topical term or geographic name as entry element |
Backcrossing |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
12563 |
| Topical term or geographic name as entry element |
PCR |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
2185 |
| Topical term or geographic name as entry element |
Alleles |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Plant diseases |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1206 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
8730 |
| Topical term or geographic name as entry element |
Introgression |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
9175 |
| Personal name |
Ifie, B.E. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
6758 |
| Personal name |
Danquah, E. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
962 |
| Personal name |
Jumbo, M.B. |
| Field link and sequence number |
INT3488 |
| Miscellaneous information |
Global Maize Program |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mahabaleswara, S.L. |
| Miscellaneous information |
Global Maize Program |
| Field link and sequence number |
I1706749 |
| 9 (RLIN) |
2827 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
795 |
| Personal name |
Gowda, M. |
| Field link and sequence number |
I1705963 |
| Miscellaneous information |
Global Maize Program |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
9176 |
| Personal name |
Marchelo-D’ragga, P.W. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
923 |
| Personal name |
Olsen, M. |
| Field link and sequence number |
INT3333 |
| Miscellaneous information |
Global Maize Program |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
20216 |
| Personal name |
Shorinola, O. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
4314 |
| Personal name |
Yao, N. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Prasanna, B.M. |
| Miscellaneous information |
Global Maize Program |
| Field link and sequence number |
INT3057 |
| 9 (RLIN) |
887 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Tongoona, P.B. |
| Field link and sequence number |
001713456 |
| Miscellaneous information |
Formerly Excellence in Breeding |
| 9 (RLIN) |
340 |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Frontiers in Plant Science |
| Related parts |
v. 12, art. 649308 |
| Place, publisher, and date of publication |
Switzerland : Frontiers, 2021. |
| International Standard Serial Number |
1664-462X |
| Record control number |
u56875 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
|---|
| Link text |
Open Access through DSpace |
| Uniform Resource Identifier |
https://hdl.handle.net/10883/21545 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Suppress in OPAC |
No |
| Source of classification or shelving scheme |
Dewey Decimal Classification |