MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03774nab|a22004817a|4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
62940 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20231018183733.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
201118s2020||||xxu|||p|op||||00||0|eng|d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
1932-6203 (Online) |
| 024 8# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
https://doi.org/10.1371/journal.pone.0238724 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Tembo, B. |
| 9 (RLIN) |
17264 |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Detection and characterization of fungus (Magnaporthe oryzae pathotype Triticum) causing wheat blast disease on rain-fed grown wheat (Triticum aestivum L.) in Zambia |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
San Francisco, CA (USA) : |
| Name of publisher, distributor, etc. |
Public Library of Science, |
| Date of publication, distribution, etc. |
2020. |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer review |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Open Access |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Wheat blast caused by Magnaporthe oryzae pathotype Triticum (MoT) is a threat to wheat production especially in the warmer-humid environments. In Zambia, wheat blast symptoms were observed for the first time on wheat (Triticum aestivum L.) grown in experimental plots and five farmers’ fields in Mpika district of Muchinga Province during the 2017–18 rainy season. Infected plants showed the typical wheat blast symptoms with the spike becoming partially or completely bleached with the blackening of the rachis in a short span of time. Incidence of blast symptoms on nearly all wheat heads was high and ranged from 50 to 100%. Examination of diseased plant leaves showed the presence of elliptical, grayish to tan necrotic lesions with dark borders on the leaf often mixed with other foliar diseases. A study was conducted to isolate and identify the causal pathogen(s) using classical and molecular methods and determine the pathogenicity of the detected disease causal agent. Morphobiometrical determination of causal pathogen revealed conidia with characteristic pear shaped 2-septate hyaline spores associated with M. oryzae species. Preliminary polymerase chain reaction screening of six isolates obtained from wheat blast infected samples with diagnostic primers (MoT3F/R) was conducted at ZARI, Zambia, and subsequent analysis of two isolates with MoT3F/R and C17F/R was performed at USDA-ARS, USA. Both experiments confirmed that MoT is the causal agent of wheat blast in Zambia. Further, pathogenicity tests performed with pure culture isolates from samples WS4 and WS5 produced typical blast symptoms on all the six inoculated wheat genotypes. Results of this study indicate that MoT is causing wheat blast in rain-fed wheat grown in Zambia, thus making it the first report of MoT in Zambia and Africa. This inter-continental movement of the pathogen (disease) has serious implication for wheat production and trade that needs to be urgently addressed. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Wheat |
| Miscellaneous information |
AGROVOC |
| Source of heading or term |
|
| 9 (RLIN) |
1310 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
PCR |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
12563 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Fungi |
| Miscellaneous information |
AGROVOC |
| Source of heading or term |
|
| 9 (RLIN) |
1121 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Epidemiology |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
6627 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Fungal spores |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
17265 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Pathogenesis |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
4080 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Leaves |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
8838 |
| 651 #7 - SUBJECT ADDED ENTRY--GEOGRAPHIC NAME |
|---|
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
4309 |
| Geographic name |
Zambia |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Rabson M. Mulenga |
| 9 (RLIN) |
13646 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Sichilima, S. |
| 9 (RLIN) |
17266 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
M’siska, K.K. |
| 9 (RLIN) |
17267 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mwale, M. |
| 9 (RLIN) |
17268 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Chikoti, P.C. |
| 9 (RLIN) |
17269 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Pawan Kumar Singh |
| Miscellaneous information |
Global Wheat Program |
| Field link and sequence number |
INT2868 |
| 9 (RLIN) |
868 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Xinyao He |
| 9 (RLIN) |
913 |
| Field link and sequence number |
INT3297 |
| Miscellaneous information |
Global Wheat Program |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Pedley, K.F. |
| 9 (RLIN) |
4214 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Peterson, G.L. |
| 9 (RLIN) |
4213 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Singh, R.P. |
| Miscellaneous information |
Global Wheat Program |
| Field link and sequence number |
INT0610 |
| 9 (RLIN) |
825 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Braun, H.J. |
| Miscellaneous information |
Formerly Global Wheat Program |
| Field link and sequence number |
INT0599 |
| 9 (RLIN) |
824 |
| 773 0# - HOST ITEM ENTRY |
|---|
| Related parts |
v. 15, no. 9, e0238724 |
| Place, publisher, and date of publication |
San Francisco, CA (USA) : Public Library of Science, 2020. |
| Title |
PLoS One |
| International Standard Serial Number |
1932-6203 |
| Record control number |
u94957 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
|---|
| Link text |
Open Access through DSpace |
| Uniform Resource Identifier |
https://hdl.handle.net/10883/21036 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Suppress in OPAC |
No |
| Source of classification or shelving scheme |
Dewey Decimal Classification |