Knowledge Center Catalog

Detection and characterization of fungus (Magnaporthe oryzae pathotype Triticum) causing wheat blast disease on rain-fed grown wheat (Triticum aestivum L.) in Zambia (Record no. 62940)

MARC details
000 -LEADER
fixed length control field 03774nab|a22004817a|4500
001 - CONTROL NUMBER
control field 62940
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20231018183733.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 201118s2020||||xxu|||p|op||||00||0|eng|d
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 1932-6203 (Online)
024 8# - OTHER STANDARD IDENTIFIER
Standard number or code https://doi.org/10.1371/journal.pone.0238724
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Tembo, B.
9 (RLIN) 17264
245 10 - TITLE STATEMENT
Title Detection and characterization of fungus (Magnaporthe oryzae pathotype Triticum) causing wheat blast disease on rain-fed grown wheat (Triticum aestivum L.) in Zambia
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. San Francisco, CA (USA) :
Name of publisher, distributor, etc. Public Library of Science,
Date of publication, distribution, etc. 2020.
500 ## - GENERAL NOTE
General note Peer review
500 ## - GENERAL NOTE
General note Open Access
520 ## - SUMMARY, ETC.
Summary, etc. Wheat blast caused by Magnaporthe oryzae pathotype Triticum (MoT) is a threat to wheat production especially in the warmer-humid environments. In Zambia, wheat blast symptoms were observed for the first time on wheat (Triticum aestivum L.) grown in experimental plots and five farmers’ fields in Mpika district of Muchinga Province during the 2017–18 rainy season. Infected plants showed the typical wheat blast symptoms with the spike becoming partially or completely bleached with the blackening of the rachis in a short span of time. Incidence of blast symptoms on nearly all wheat heads was high and ranged from 50 to 100%. Examination of diseased plant leaves showed the presence of elliptical, grayish to tan necrotic lesions with dark borders on the leaf often mixed with other foliar diseases. A study was conducted to isolate and identify the causal pathogen(s) using classical and molecular methods and determine the pathogenicity of the detected disease causal agent. Morphobiometrical determination of causal pathogen revealed conidia with characteristic pear shaped 2-septate hyaline spores associated with M. oryzae species. Preliminary polymerase chain reaction screening of six isolates obtained from wheat blast infected samples with diagnostic primers (MoT3F/R) was conducted at ZARI, Zambia, and subsequent analysis of two isolates with MoT3F/R and C17F/R was performed at USDA-ARS, USA. Both experiments confirmed that MoT is the causal agent of wheat blast in Zambia. Further, pathogenicity tests performed with pure culture isolates from samples WS4 and WS5 produced typical blast symptoms on all the six inoculated wheat genotypes. Results of this study indicate that MoT is causing wheat blast in rain-fed wheat grown in Zambia, thus making it the first report of MoT in Zambia and Africa. This inter-continental movement of the pathogen (disease) has serious implication for wheat production and trade that needs to be urgently addressed.
546 ## - LANGUAGE NOTE
Language note Text in English
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Wheat
Miscellaneous information AGROVOC
Source of heading or term
9 (RLIN) 1310
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element PCR
Source of heading or term AGROVOC
9 (RLIN) 12563
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Fungi
Miscellaneous information AGROVOC
Source of heading or term
9 (RLIN) 1121
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Epidemiology
Source of heading or term AGROVOC
9 (RLIN) 6627
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Fungal spores
Source of heading or term AGROVOC
9 (RLIN) 17265
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Pathogenesis
Source of heading or term AGROVOC
9 (RLIN) 4080
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Leaves
Source of heading or term AGROVOC
9 (RLIN) 8838
651 #7 - SUBJECT ADDED ENTRY--GEOGRAPHIC NAME
Source of heading or term AGROVOC
9 (RLIN) 4309
Geographic name Zambia
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Rabson M. Mulenga
9 (RLIN) 13646
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Sichilima, S.
9 (RLIN) 17266
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name M’siska, K.K.
9 (RLIN) 17267
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Mwale, M.
9 (RLIN) 17268
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Chikoti, P.C.
9 (RLIN) 17269
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Pawan Kumar Singh
Miscellaneous information Global Wheat Program
Field link and sequence number INT2868
9 (RLIN) 868
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Xinyao He
9 (RLIN) 913
Field link and sequence number INT3297
Miscellaneous information Global Wheat Program
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Pedley, K.F.
9 (RLIN) 4214
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Peterson, G.L.
9 (RLIN) 4213
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Singh, R.P.
Miscellaneous information Global Wheat Program
Field link and sequence number INT0610
9 (RLIN) 825
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Braun, H.J.
Miscellaneous information Formerly Global Wheat Program
Field link and sequence number INT0599
9 (RLIN) 824
773 0# - HOST ITEM ENTRY
Related parts v. 15, no. 9, e0238724
Place, publisher, and date of publication San Francisco, CA (USA) : Public Library of Science, 2020.
Title PLoS One
International Standard Serial Number 1932-6203
Record control number u94957
856 4# - ELECTRONIC LOCATION AND ACCESS
Link text Open Access through DSpace
Uniform Resource Identifier https://hdl.handle.net/10883/21036
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Suppress in OPAC No
Source of classification or shelving scheme Dewey Decimal Classification
Holdings
Date last seen Total Checkouts Price effective from Koha item type Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
11/27/2020   11/27/2020 Article Not Lost Dewey Decimal Classification     CIMMYT Staff Publications Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 11/27/2020

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