MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02757nab|a22003377a|4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
62729 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20240919021229.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
200423s2020||||xxk|||p|op||||00||0|eng|d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
2045-2322 |
| 024 8# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
https://doi.org/10.1038/s41598-020-59580-5 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Garrido, J. |
| 9 (RLIN) |
16486 |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Identification and validation of reference genes for RT-qPCR normalization in wheat meiosis |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
London (United Kingdom) : |
| Name of publisher, distributor, etc. |
Nature Publishing Group, |
| Date of publication, distribution, etc. |
2020. |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer review |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Open Access |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Meiosis is a specialized type of cell division occurring in sexually reproducing organisms to generate haploid cells known as gametes. In flowering plants, male gametes are produced in anthers, being encased in pollen grains. Understanding the genetic regulation of meiosis key events such as chromosome recognition and pairing, synapsis and recombination, is needed to manipulate chromosome associations for breeding purposes, particularly in important cereal crops like wheat. Reverse transcription-quantitative PCR (RT-qPCR) is widely used to analyse gene expression and to validate the results obtained by other transcriptomic analyses, like RNA-seq. Selection and validation of appropriate reference genes for RT-qPCR normalization is essential to obtain reproducible and accurate expression data. In this work, twelve candidate reference genes were evaluated using the mainstream algorithms geNorm, Normfinder, BestKeeper and ΔCt, then ranked from most to least suitable for normalization with RefFinder. Different sets of reference genes were recommended to normalize gene expression data in anther meiosis of bread and durum wheat, their corresponding genotypes in the absence of the Ph1 locus and for comparative studies among wheat genotypes. Comparisons between meiotic (anthers) and somatic (leaves and roots) wheat tissues were also carried out. To the best of our knowledge, our study provides the first comprehensive list of reference genes for robust RT-qPCR normalization to study differentially expressed genes during male meiosis in wheat in a breeding framework. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Wheat |
| Miscellaneous information |
AGROVOC |
| Source of heading or term |
|
| 9 (RLIN) |
1310 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
8835 |
| Topical term or geographic name as entry element |
Gene Expression |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Plant breeding |
| Miscellaneous information |
AGROVOC |
| Source of heading or term |
|
| 9 (RLIN) |
1203 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
16487 |
| Topical term or geographic name as entry element |
Reverse transcription |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
12563 |
| Topical term or geographic name as entry element |
PCR |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Aguilar, M. |
| 9 (RLIN) |
16488 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Prieto, P. |
| 9 (RLIN) |
16489 |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Nature Scientific Reports |
| Related parts |
v. 10, art. 2726 |
| Place, publisher, and date of publication |
London (United Kingdom) : Nature Publishing Group, 2020. |
| International Standard Serial Number |
2045-2322 |
| Record control number |
a58025 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
|---|
| Link text |
Click here to access online |
| Uniform Resource Identifier |
https://doi.org/10.1038/s41598-020-59580-5 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Suppress in OPAC |
No |
| Source of classification or shelving scheme |
Dewey Decimal Classification |