Novel ASR isolated from drought stress responsive SSH library in pearl millet confers multiple abiotic stress tolerance in PgASR3 transgenic Arabidopsis (Record no. 62556)
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| 000 -LEADER | |
|---|---|
| fixed length control field | nab a22 7a 4500 |
| 001 - CONTROL NUMBER | |
| control field | 62556 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20200911205319.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200602s2020 ne |||p|op||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 0981-9428 |
| 024 8# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | https://doi.org/10.1016/j.plaphy.2020.07.031 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MX-TxCIM |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 15763 |
| Personal name | Meena, R.P. |
| 245 10 - TITLE STATEMENT | |
| Title | Novel ASR isolated from drought stress responsive SSH library in pearl millet confers multiple abiotic stress tolerance in PgASR3 transgenic Arabidopsis |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Amsterdam (Netherlands) : |
| Name of publisher, distributor, etc. | Elsevier, |
| Date of publication, distribution, etc. | 2020. |
| 500 ## - GENERAL NOTE | |
| General note | Peer review |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | A genomic resource of drought stress responsive genes/ESTs was generated using Suppression Subtractive Hybridization (SSH) approach in a drought stress tolerant Pennisetum glaucum genotype 841B. Fifty five days old plants were subjected to drought stress after withholding water for different time intervals (10 days, 15 days, 20 days and 25 days). A forward subtractive cDNA library was prepared from isolated RNA of leaf tissue. Differential gene expression under drought stress was validated for selected nine contigs by RT-qPCR. A transcript homologous to Setaria italica ASR3 upregulated under drought stress was isolated from genotype 841B and characterized. Heterologous expression of PgASR3 was validated in Arabidopsis and confirmed under multiple abiotic stress conditions. A total of four independent transgenic lines overexpressing gene PgASR3 were analyzed by Southern blot at T1 stage. For drought stress tolerance, three independent lines (T2 stage) were analyzed by biochemical and physiological assays at seedling stage. The growth rate (shoot and root length) of transgenic seedlings improved as compared to WT seedling under differenct abiotic stress conditions. The three transgenic lines were also validated for drought stress tolerance and RT-qPCR analysis, at maturity stage. Under drought stress conditions, the mature transgenic lines showed higher levels of RWC, chlorophyll and proline but lower levels of MDA as compared to WT plants. PgASR3 gene isolated and validated in this study can be utilized for developing abiotic stress tolerant crops. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 3448 |
| Topical term or geographic name as entry element | Abiotic stress |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 1081 |
| Topical term or geographic name as entry element | Drought stress |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 2433 |
| Topical term or geographic name as entry element | Pennisetum glaucum |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 15764 |
| Topical term or geographic name as entry element | Transgenics |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 15765 |
| Personal name | Vishwakarma, H. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 15766 |
| Personal name | Ghosh, G. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 15767 |
| Personal name | Gaikwad, K. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 15768 |
| Personal name | Chellapilla, T.S. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 15769 |
| Personal name | Singh, M.P |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 15770 |
| Personal name | Padaria, J.C. |
| 773 0# - HOST ITEM ENTRY | |
| Place, publisher, and date of publication | Amsterdam (Netherlands) : Elsevier, 2020. |
| Related parts | v. 156, p. 7-19 |
| Title | Plant Physiology and Biochemistry |
| International Standard Serial Number | 0981-9428 |
| Record control number | u91708 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Article |
| Suppress in OPAC | No |
| Date last seen | Total Checkouts | Price effective from | Koha item type | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Withdrawn status | Home library | Current library | Date acquired |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 09/11/2020 | 09/11/2020 | Article | Not Lost | Dewey Decimal Classification | Reprints Collection | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Knowledge Center: John Woolston Library | 09/11/2020 |