Specific identification and detection of Pantoea stewartii subsp. stewartii using a membrane-based multi-gene oligonucleotide array (Record no. 62222)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02156nab|a22002657a|4500 |
| 001 - CONTROL NUMBER | |
| control field | 62222 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20211006085211.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200325s2015||||xxk|||p|op||||00||0|eng|d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 0706-0661 |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 1715-2992 (Online) |
| 024 8# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | https://doi.org/10.1080/07060661.2015.1113442 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MX-TxCIM |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Tambong, J.T. |
| 9 (RLIN) | 14567 |
| 245 10 - TITLE STATEMENT | |
| Title | Specific identification and detection of Pantoea stewartii subsp. stewartii using a membrane-based multi-gene oligonucleotide array |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | United Kingdom : |
| Name of publisher, distributor, etc. | Taylor and Francis, |
| Date of publication, distribution, etc. | 2015. |
| 500 ## - GENERAL NOTE | |
| General note | Peer review |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The Stewart’s wilt pathogen (Pantoea stewartii subsp. stewartii; Pss) of sweet corn is classified as a quarantine bacterium, requiring certification of grain shipments made to over 60 countries. This study reports the development and validation of the first multi-gene array for accurate and specific differentiation of Pss from Pantoea stewartii subsp. indologenes (Psi) and other Pantoea species using DNA, or RNA after generation of cDNA. The technique consisted of multiplex (16S rRNA, leuS, gyrB, rpoB, cpsD) PCR amplifications using universal and specific primers in a single reaction, followed by the hybridization of the digoxigenin-labelled amplicons to 22 specific oligonucleotide probes (19- to 24-mers) immobilized on a nylon membrane. The sensitivity of the array (10 fg of DNA) compared favourably with TaqMan real-time PCR. The specificity and reliability of the array was tested on 65 bacterial strains consisting of Pantoea species, closely and distantly related bacterial genera. Specific detection of Pss in infected corn leaves and seed homogenates was also validated using growth chamber-inoculated plants. The reported multi-gene DNA array could be a reliable tool for routine and specific detection of Pss. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Maize |
| Miscellaneous information | AGROVOC |
| Source of heading or term | |
| 9 (RLIN) | 1173 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 14568 |
| Topical term or geographic name as entry element | Pantoea stewartii |
| 773 0# - HOST ITEM ENTRY | |
| Title | Canadian Journal of Plant Pathology |
| Related parts | v. 37, no. 4, p. 414-426 |
| Place, publisher, and date of publication | United Kingdom : Taylor and Francis, 2015. |
| International Standard Serial Number | 0706-0661 |
| Record control number | u444208 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Article |
| Suppress in OPAC | No |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Date last seen | Total Checkouts | Price effective from | Koha item type | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Withdrawn status | Home library | Current library | Date acquired |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 07/06/2020 | 07/06/2020 | Article | Not Lost | Dewey Decimal Classification | Reprints Collection | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Knowledge Center: John Woolston Library | 07/06/2020 |