Towards identification of molecular mechanism in which the overexpression of wheat cytosolic and plastid glutamine synthetases in tobacco enhanced drought tolerance (Record no. 62138)
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| 000 -LEADER | |
|---|---|
| fixed length control field | nab a22 7a 4500 |
| 001 - CONTROL NUMBER | |
| control field | 62138 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20200629211301.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200618s2020 ne |||p|op||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 0981-9428 |
| 024 8# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | https://doi.org/10.1016/j.plaphy.2020.04.013 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MX-TxCIM |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 14269 |
| Personal name | Haidong Yu |
| 245 10 - TITLE STATEMENT | |
| Title | Towards identification of molecular mechanism in which the overexpression of wheat cytosolic and plastid glutamine synthetases in tobacco enhanced drought tolerance |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Amsterdam (Netherlands) : |
| Name of publisher, distributor, etc. | Elsevier, |
| Date of publication, distribution, etc. | 2020. |
| 500 ## - GENERAL NOTE | |
| General note | Peer review |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Glutamine synthetases (GS) play an essential role in Nitrogen assimilation. Nonetheless, information respecting the molecular functions of GS in drought tolerance (DT) is limited. Here we show that overexpressing cytosolic GS1 or plastidic GS2 gene in tobacco enhanced DT of both root and leaf tissues of the two transgenic seedlings (named as GS1-TR and GS2-TR). RNA-seq analysis on root tissues showed that 83 aquaporin (AQP) genes were identified. Among them, 37 differential expression genes (DEGs) were found in the GS1-TR roots under normal condition, and all were down-regulated; no any DEGs in the GS2-TR roots were found. Contrastingly, under drought, 28 and 32 DEGs of AQP were up-regulated in GS1-TR and GS2-TR roots, respectively. GC-MS analysis on leaf tissues showed that glutamine (Gln) concentrations were negatively correlated AQP expressions in the all four conditions, which suggests that Gln, as a signal molecule, can negatively regulate many AQP expressions. Prestress accumulation of sucrose and proline (Pro) and chlorophyll, and had higher activities of ROS scavengers also contribute the plant DT in both of the two transgenic plants under drought. In addition, 5-aminolevulinic acid (ALA) was up-accumulated in GS2-TR leaves solely under normal condition, which leads to its net photosynthetic rate higher than that in GS1-TR leaves. Last but not the less, the PYL-PP2C-SnRK2 core ABA-signaling pathway was uniquely activated in GS1-TR independent of drought stress (DS). Therefore, our results suggest a possible model reflecting how overexpression of wheat TaGS1 and TaGS2 regulate plant responses to drought. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 14270 |
| Topical term or geographic name as entry element | Glutamate ammonia ligase |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 14271 |
| Topical term or geographic name as entry element | Glutamine |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 6733 |
| Topical term or geographic name as entry element | Nitrogen metabolism |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 3517 |
| Topical term or geographic name as entry element | ABA |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 1082 |
| Topical term or geographic name as entry element | Drought tolerance |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 14272 |
| Personal name | Yiming Zhang |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 14273 |
| Personal name | Zhiyong Zhang |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 14274 |
| Personal name | Jie Zhang |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 14275 |
| Personal name | Yihao Wei |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 14276 |
| Personal name | Xiting Jia |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 14277 |
| Personal name | Xiaochun Wang |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 14278 |
| Personal name | Xinming Ma |
| 773 0# - HOST ITEM ENTRY | |
| Place, publisher, and date of publication | Amsterdam (Netherlands) : Elsevier, 2020. |
| Related parts | v. 151, p. 608-620 |
| Title | Plant Physiology and Biochemistry |
| International Standard Serial Number | 0981-9428 |
| Record control number | u91708 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Article |
| Suppress in OPAC | No |
| Date last seen | Total Checkouts | Price effective from | Koha item type | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Withdrawn status | Home library | Current library | Date acquired |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 06/19/2020 | 06/19/2020 | Article | Not Lost | Dewey Decimal Classification | Reprints Collection | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Knowledge Center: John Woolston Library | 06/19/2020 |