MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02665nab a22003257a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
62063 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20230425210810.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
200602s2020 ne |||p|op||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
0141-8130 |
| 024 8# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
https://doi.org/10.1016/j.ijbiomac.2020.04.069 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
13919 |
| Personal name |
Kummari, D. |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Functional characterization of the promoter of pearl millet heat shock protein 10 (PgHsp10) in response to abiotic stresses in transgenic tobacco plants |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
Amsterdam (Netherlands) : |
| Name of publisher, distributor, etc. |
Elsevier, |
| Date of publication, distribution, etc. |
2020. |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer review |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
In the present study, the promoter region of the pearl millet heat shock protein 10 (PgHsp10) gene was cloned and characterized. The PgHsp10 promoter (PgHsp10pro) sequence region has all the cis-motifs required for tissue and abiotic stress inducibility. The complete PgHsp10pro (PgHsp10PC) region and a series of 5′ truncations of PgHsp10 (PgHsp10D1 and PgHsp10D2) and an antisense form of PgHsp10pro (PgHsp10AS) were cloned into a plant expression vector (pMDC164) through gateway cloning. All four constructs were separately transformed into tobacco through Agrobacterium-mediated genetic transformation, and PCR-confirmed transgenic plants progressed to T1 and T2 generations. The T2 transgenic tobacco plants comprising all PgHsp10pro fragments were used for GUS histochemical and qRT-PCR assays in different tissues under control and abiotic stresses. The PgHsp10PC pro expression was specific to stem and seedlings under control conditions. Under different abiotic stresses, particularly heat stress, PgHsp10PCpro had relatively higher activity than PgHsp10D1pro, PgHsp10D2pro and PgHsp10ASpro. PgHsp10pro from a stress resilient crop like pearl millet responds positively to a range of abiotic stresses, in particular heat, when expressed in heterologous plant systems such as tobacco. Hence, PgHsp10pro appears to be a potential promoter candidate for developing heat and drought stress-tolerant crop plants. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
13920 |
| Topical term or geographic name as entry element |
Pearl millet |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
2433 |
| Topical term or geographic name as entry element |
Pennisetum glaucum |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
3448 |
| Topical term or geographic name as entry element |
Abiotic stress |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1971 |
| Topical term or geographic name as entry element |
Heat stress |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Bhatnagar-Mathur, P. |
| Field link and sequence number |
001712891 |
| Miscellaneous information |
Formerly Genetic Resources Program |
| 9 (RLIN) |
13921 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
13922 |
| Personal name |
Sharma, K.K. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
3886 |
| Personal name |
Vadez, V. |
| Field link and sequence number |
001712453 |
| Miscellaneous information |
Formerly Excellence in Breeding |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
13923 |
| Personal name |
Palakolanu, S.R. |
| 773 0# - HOST ITEM ENTRY |
|---|
| Place, publisher, and date of publication |
Amsterdam (Netherlands) : Elsevier, 2018. |
| Related parts |
v. 156, p. 103-110 |
| Title |
International Journal of Biological Macromolecules |
| International Standard Serial Number |
0141-8130 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Article |
| Suppress in OPAC |
No |