Knowledge Center Catalog

High-resolution melting analysis for SNP genotyping and mapping in tetraploid alfalfa (Medicago sativa L.) (Record no. 61800)

MARC details
000 -LEADER
fixed length control field 02409nab|a22003257a|4500
001 - CONTROL NUMBER
control field 61800
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250923171408.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 200503s2012||||ne |||p|op||||00||0|eng|d
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 1380-3743
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 1572-9788 (Online)
024 8# - OTHER STANDARD IDENTIFIER
Standard number or code https://doi.org/10.1007/s11032-011-9566-x
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Yuanhong Han
9 (RLIN) 12797
245 10 - TITLE STATEMENT
Title High-resolution melting analysis for SNP genotyping and mapping in tetraploid alfalfa (Medicago sativa L.)
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. Dordrecht (Netherlands) :
Name of publisher, distributor, etc. Springer,
Date of publication, distribution, etc. 2012.
500 ## - GENERAL NOTE
General note Peer review
500 ## - GENERAL NOTE
General note Open Access
520 ## - SUMMARY, ETC.
Summary, etc. Single nucleotide polymorphisms (SNPs) represent the most abundant type of genetic polymorphism in plant genomes. SNP markers are valuable tools for genetic analysis of complex traits of agronomic importance, linkage and association mapping, genome-wide selection, map-based cloning, and marker-assisted selection. Current challenges for SNP genotyping in polyploid outcrossing species include multiple alleles per loci and lack of high-throughput methods suitable for variant detection. In this study, we report on a high-resolution melting (HRM) analysis system for SNP genotyping and mapping in outcrossing tetraploid genotypes. The sensitivity and utility of this technology is demonstrated by identification of the parental genotypes and segregating progeny in six alfalfa populations based on unique melting curve profiles due to differences in allelic composition at one or multiple loci. HRM using a 384-well format is a fast, consistent, and efficient approach for SNP discovery and genotyping, useful in polyploid species with uncharacterized genomes. Possible applications of this method include variation discovery, analysis of candidate genes, genotyping for comparative and association mapping, and integration of genome-wide selection in breeding programs.
546 ## - LANGUAGE NOTE
Language note Text in English
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Medicago sativa
Miscellaneous information AGROVOC
9 (RLIN) 38417
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Source of heading or term AGROVOC
9 (RLIN) 2084
Topical term or geographic name as entry element Chromosome mapping
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Single nucleotide polymorphisms
Miscellaneous information AGROVOC
9 (RLIN) 10805
700 0# - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 12798
Personal name Dong-Man Khu
700 1# - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 12799
Personal name Monteros, M.J.
773 0# - HOST ITEM ENTRY
Title Molecular Breeding
Related parts v. 29, no. 2, p. 489-501
Place, publisher, and date of publication Dordrecht (Netherlands) : Springer, 2012.
International Standard Serial Number 1380-3743
Record control number u78961
856 4# - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier https://doi.org/10.1007/s11032-011-9566-x
Link text Click here to access online
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Suppress in OPAC No
Source of classification or shelving scheme Dewey Decimal Classification
Holdings
Date last seen Total Checkouts Price effective from Koha item type Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
05/05/2020   05/05/2020 Article Not Lost Dewey Decimal Classification     Reprints Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 05/05/2020

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