Identification of Clostridium beijerinckii, Cl. butyricum, Cl. sporogenes, Cl. tyrobutyricum isolated from silage, raw milk and hard cheese by a multiplex PCR assay (Record no. 61775)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 00595nab|a22002177a|4500 |
| 001 - CONTROL NUMBER | |
| control field | 61775 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20200925164639.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200423s2012||||xxk|||p|op||||00||0|eng|d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 0022-0299 |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 1469-7629 (Online) |
| 024 8# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | https://doi.org/10.1017/S002202991200026X |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MX-TxCIM |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 12683 |
| Personal name | Cremonesi, P. |
| 245 10 - TITLE STATEMENT | |
| Title | Identification of Clostridium beijerinckii, Cl. butyricum, Cl. sporogenes, Cl. tyrobutyricum isolated from silage, raw milk and hard cheese by a multiplex PCR assay |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Cambridge (United Kingdom) : |
| Name of publisher, distributor, etc. | Cambridge University Press, |
| Date of publication, distribution, etc. | 2012. |
| 500 ## - GENERAL NOTE | |
| General note | Peer review |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Late blowing, caused by the outgrowth of clostridial spores present in raw milk and originating from silage, can create considerable product loss, especially in the production of hard and semi-hard cheeses. The conventional method for the isolation of Clostridium spp. from cheeses with late-blowing symptoms is very complicated and the identification of isolates is problematic. The aim of this work was the development of a multiplex PCR method for the detection of the main dairy-related clostridia such as: Cl. beijerinckii, Cl. butyricum, Cl. sporogenes, Cl. tyrobutyricum. Samples derived from silage, raw milk and hard cheese were analysed by the most probable number (MPN) enumeration. Forty-four bacterial strains isolated from gas positive tubes were used to check the reliability of the multiplex PCR assay. The specificity of the primers was tested by individually analysing each primer pair and the primer pair combined in the multiplex PCR. It was interesting to note that the samples not identified by the multiplex PCR assay were amplified by V2–V3 16S rRNA primer pair and the sequencing revealed the aligned 16S rRNA sequences to be Paenibacillus and Bacillus spp. This new molecular assay provides a simple promising alternative to traditional microbiological methods for a rapid, sensitive detection of clostridia in dairy products. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 12684 |
| Topical term or geographic name as entry element | Clostridium |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 12685 |
| Topical term or geographic name as entry element | Raw milk |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 10374 |
| Topical term or geographic name as entry element | Silage |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Source of heading or term | AGROVOC |
| 9 (RLIN) | 8681 |
| Topical term or geographic name as entry element | Cheese |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 12686 |
| Personal name | Vanoni, L. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 12687 |
| Personal name | Silvetti, T. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 12688 |
| Personal name | Morandi, S. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 12689 |
| Personal name | Brasca, M. |
| 773 0# - HOST ITEM ENTRY | |
| Title | Journal of Dairy Research |
| Related parts | v. 79, no. 3, p. 318-323 |
| Place, publisher, and date of publication | Cambridge (United Kingdom) : Cambridge University Press, 2012. |
| International Standard Serial Number | 0022-0299 |
| Record control number | u76377 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Article |
| Suppress in OPAC | No |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Date last seen | Total Checkouts | Price effective from | Koha item type | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Withdrawn status | Home library | Current library | Date acquired |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 04/30/2020 | 04/30/2020 | Article | Not Lost | Dewey Decimal Classification | Reprints Collection | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Knowledge Center: John Woolston Library | 04/30/2020 |