Knowledge Center Catalog

Species‐specific real‐time PCR detection of Colletotrichum kahawae (Record no. 61734)

MARC details
000 -LEADER
fixed length control field 02438nab|a22003257a|4500
001 - CONTROL NUMBER
control field 61734
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20211006073554.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 200423s2013||||xxk|||p|op||||00||0|eng|d
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 1364-5072
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 1365-2672 (Online)
024 8# - OTHER STANDARD IDENTIFIER
Standard number or code https://doi.org/10.1111/jam.12068
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Tao, G.
9 (RLIN) 12543
245 10 - TITLE STATEMENT
Title Species‐specific real‐time PCR detection of Colletotrichum kahawae
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. United Kingdom :
Name of publisher, distributor, etc. Wiley,
Date of publication, distribution, etc. 2013.
500 ## - GENERAL NOTE
General note Peer review
520 ## - SUMMARY, ETC.
Summary, etc. Aims. Colletotrichum kahawae is a strongly aggressive pathogen causing coffee berry disease and is specific to Arabica coffee (Coffea arabica) in Africa. In this article, we developed a real‐time PCR assay for the species‐specific diagnosis of C. kahawae by designing the primers and a TaqMan probe derived from the single nucleotide polymorphism‐rich region of glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) gene. Methods and Results. DNA markers from rDNA internal transcribed spacer, actin, β‐tubulin and GAPDH genes of the ex‐type culture of C. kahawae and 10 reference strains of Colletotrichum species were analysed for intra‐ and interspecific variations. The GAPDH gene was selected to develop a species‐specific DNA marker. A TaqMan real‐time PCR assay for species‐specific detection of C. kahawae was developed, and its accuracy was tested against type strains of other phylogenetically closely related species in the C. gloeosporioides species complex, with the detection sensitivity of 80 fg μl−1 of genomic DNA. Conclusions. This real‐time PCR assay is highly specific and sensitive for the diagnosis of C. kahawae and can be applied in qualitative and quantitative tests. Significance and Impact of the Study. This protocol allows for a rapid and sensitive detection of C. kahawae and will be useful in disease management and pest detection to prevent further spread of this pathogen.
546 ## - LANGUAGE NOTE
Language note Text in English
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Source of heading or term AGROVOC
9 (RLIN) 11096
Topical term or geographic name as entry element Coffea arabica
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Source of heading or term AGROVOC
9 (RLIN) 12544
Topical term or geographic name as entry element Glomerella cingulata
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Source of heading or term AGROVOC
9 (RLIN) 5807
Topical term or geographic name as entry element Diagnosis
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Plant diseases
Miscellaneous information AGROVOC
Source of heading or term
9 (RLIN) 1206
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Source of heading or term AGROVOC
9 (RLIN) 3563
Topical term or geographic name as entry element Genes
700 1# - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 12545
Personal name Hyde, K.D.
700 0# - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 12546
Personal name Lei Cai
773 0# - HOST ITEM ENTRY
Title Journal of Applied Microbiology
Related parts v. 114, no. 3, p. 828-835
Place, publisher, and date of publication United Kingdom : Wiley, 2013.
International Standard Serial Number 1365-2672
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Suppress in OPAC No
Source of classification or shelving scheme Dewey Decimal Classification
Holdings
Date last seen Total Checkouts Price effective from Koha item type Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
04/27/2020   04/27/2020 Article Not Lost Dewey Decimal Classification     Reprints Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 04/27/2020

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