Knowledge Center Catalog

Denaturing gradient gel electrophoresis gel expansion (DGGEGE) – An attempt to resolve the limitations of co-migration in the DGGE of complex polymicrobial communities (Record no. 61689)

MARC details
000 -LEADER
fixed length control field 01817nab a22002897a 4500
001 - CONTROL NUMBER
control field 61689
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20230313165819.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 200410s2005||||xxk|||p|op||||00||0|eng|d
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 0378-1097
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 1574-6968 (Online)
024 8# - OTHER STANDARD IDENTIFIER
Standard number or code https://doi.org/10.1016/j.femsle.2005.09.048
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Gafan, G.P.
9 (RLIN) 12356
245 10 - TITLE STATEMENT
Title Denaturing gradient gel electrophoresis gel expansion (DGGEGE) – An attempt to resolve the limitations of co-migration in the DGGE of complex polymicrobial communities
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. United Kingdom :
Name of publisher, distributor, etc. Oxford University Press,
Date of publication, distribution, etc. 2005.
500 ## - GENERAL NOTE
General note Peer review
520 ## - SUMMARY, ETC.
Summary, etc. Recent molecular approaches for the study of microbial communities such as PCR-cloning have enabled the detection and identification of as-yet-unculturable taxa. Cloning and sequencing of multiple samples is extremely laborious and expensive to perform thoroughly due to the large diversity involved. For this purpose, techniques such as denaturing gradient gel electrophoresis (DGGE) may be better suited. There is increasing evidence suggesting that DGGE of complex polymicrobial communities may be limited by co-migration of different sequences. In this study, we attempt to address this limitation by excising individual bands and running them through a shorter denaturant gradient, a process we have termed “denaturing gradient gel electrophoresis gel expansion” (DGGEGE).
546 ## - LANGUAGE NOTE
Language note Text in English
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Source of heading or term AGROVOC
9 (RLIN) 12357
Topical term or geographic name as entry element Denaturation
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Source of heading or term AGROVOC
9 (RLIN) 6243
Topical term or geographic name as entry element Ribosomal DNA
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Microorganisms
Miscellaneous information AGROVOC
9 (RLIN) 10080
700 1# - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 12358
Personal name Spratt, D.A.
773 0# - HOST ITEM ENTRY
Title FEMS Microbiology Letters
Related parts v. 253, no. 2, p. 303-307
Place, publisher, and date of publication United Kingdom : Oxford University Press, 2005.
International Standard Serial Number 1574-6968
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Suppress in OPAC No
Source of classification or shelving scheme Dewey Decimal Classification
Holdings
Date last seen Total Checkouts Price effective from Koha item type Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
04/20/2020   04/20/2020 Article Not Lost Dewey Decimal Classification     Reprints Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 04/20/2020

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