MARC details
| 000 -LEADER |
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| fixed length control field |
03538nab a22004097a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
58741 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20211006085136.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
151020s2017 sz |||p|op||| 00| 0 eng d |
| 024 8# - OTHER STANDARD IDENTIFIER |
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| Standard number or code |
https://doi.org/10.3389/fpls.2017.01022 |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
CI10YT |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
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| 9 (RLIN) |
5051 |
| Personal name |
Hewei Du |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Bulked segregant RNA-seq reveals differential expression and SNPs of candidate genes associated with waterlogging tolerance in maize |
| Medium |
[Electronic Resource] |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
Switzerland : |
| Name of publisher, distributor, etc. |
Frontiers, |
| Date of publication, distribution, etc. |
2017. |
| 500 ## - GENERAL NOTE |
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| General note |
Peer review |
| 500 ## - GENERAL NOTE |
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| General note |
Open Access |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Waterlogging has increasingly become one of the major constraints to maize productivity in some maize production zones because it causes serious yield loss. Bulked segregant RNA-seq (BSR-seq) has been widely applied to profile candidate genes and map associated Single Nucleotide Polymorphism (SNP) markers in many species. In this study, 10 waterlogging sensitive and eight tolerant inbred lines were selected from 60 maize inbred lines with waterlogging response determined and preselected by the International Maize and Wheat Improvement Center (CIMMYT) from over 400 tropical maize inbred lines. BSR-seq was performed to identify differentially expressed genes and SNPs associated with waterlogging tolerance. Upon waterlogging stress, 354 and 1094 genes were differentially expressed in the tolerant and sensitive pools, respectively, compared to untreated controls. When tolerant and sensitive pools were compared, 593 genes were differentially expressed under untreated and 431 genes under waterlogged conditions, of which 122 genes overlapped. To validate the BSR-seq results, the expression levels of six genes were determined by qRT-PCR. The qRT-PCR results were consistent with BSR-seq results. Comparison of allelic polymorphism in mRNA sequences between tolerant and sensitive pools revealed 165 (normal condition) and 128 (waterlogged condition) high-probability SNPs. We found 18 overlapping SNPs with genomic positions mapped. Eighteen SNPs were contained in 18 genes, and eight and nine of 18 genes were responsive to waterlogging stress in tolerant and sensitive lines, respectively. Six alleles of the 18 originated from tolerant pool were significantly up-regulated under waterlogging, but not those from sensitive pool. Importantly, one allele (GRMZM2G055704) of the six genes was mapped between umc1619 and umc1948 on chromosome 1 where a QTL associated with waterlogging tolerance was identified in a previous research, strongly indicating that GRMZM2G055704 is a candidate gene responsive to waterlogging. Our research contributes to the knowledge of the molecular mechanism for waterlogging tolerance in maize. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| 9 (RLIN) |
9041 |
| Topical term or geographic name as entry element |
RNA Sequence |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
5053 |
| Topical term or geographic name as entry element |
Water tolerance |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Maize |
| Miscellaneous information |
AGROVOC |
| Source of heading or term |
|
| 9 (RLIN) |
1173 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
1937 |
| Topical term or geographic name as entry element |
Nucleotide sequence |
| Source of heading or term |
AGROVOC |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
5054 |
| Personal name |
Jianxiong Zhu |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
5055 |
| Personal name |
Hang Su |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
5056 |
| Personal name |
Ming Huang |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
5057 |
| Personal name |
Hongwei Wang |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
5058 |
| Personal name |
Shuangcheng Ding |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
5059 |
| Personal name |
Binglin Zhang |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
5060 |
| Personal name |
An Luo |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
5061 |
| Personal name |
Shudong Wei |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
5062 |
| Personal name |
Xiaohai Tian |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
857 |
| Personal name |
Yunbi Xu |
| Miscellaneous information |
Global Maize Program |
| Field link and sequence number |
INT2735 |
| 773 0# - HOST ITEM ENTRY |
|---|
| Record control number |
u56875 |
| International Standard Serial Number |
1664-462X |
| Place, publisher, and date of publication |
Switzerland : Frontiers |
| Title |
Frontiers in Plant Science |
| Related parts |
v. 8, no. 1022, p. 1-13 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
https://hdl.handle.net/10883/18874 |
| Link text |
Open Access through DSpace |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Article |
| Suppress in OPAC |
No |