MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03215nab a22003377a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
58548 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20211006082126.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
150723s2017 xxu|||po p||| 00| 0 eng d |
| 024 8# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
https://doi.org/10.1371/journal.pone.0171963 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
4696 |
| Personal name |
Long-Xi Yu |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Identification and validation of single nucleotide polymorphic markers linked to Ug99 stem rust resistance in spring wheat |
| Medium |
[Electronic Resource] |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
San Francisco, USA : |
| Name of publisher, distributor, etc. |
Public Library of Science, |
| Date of publication, distribution, etc. |
2017. |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Open Access |
| 500 ## - GENERAL NOTE |
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| General note |
Peer review |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Wheat stem rust (Puccinia graminis f. sp. tritici Eriks. and E. Henn.) is one of the most destructive diseases world-wide. Races belonging to Ug99 (or TTKSK) continue to cause crop losses in East Africa and threaten global wheat production. Developing and deploying wheat varieties with multiple race-specific genes or complex adult plant resistance is necessary to achieve durability. In the present study, we applied genome-wide association studies (GWAS) for identifying loci associated with the Ug99 stem rust resistance (SR) in a panel of wheat lines developed at the International Maize and Wheat Improvement Center (CIMMYT). Genotyping was carried out using the wheat 9K iSelect single nucleotide polymorphism (SNP) chip. Phenotyping was done in the field in Kenya by infection of Puccinia graminis f. sp. tritici race TTKST, the Sr24-virulent variant of Ug99. Marker-trait association identified 12 SNP markers significantly associated with resistance. Among them, 7 were mapped on five chromosomes. Markers located on chromosomes 4A and 4B overlapped with the location of the Ug99 resistance genes SrND643 and Sr37, respectively. Markers identified on 7DL were collocated with Sr25. Additional significant markers were located in the regions where no Sr gene has been reported. The chromosome location for five of the SNP markers was unknown. A BLASTN search of the NCBI database using the flanking sequences of the SNPs associated with Ug99 resistance revealed that several markers were linked to plant disease resistance analogues, while others were linked to regulatory factors or metabolic enzymes. A KASP (Kompetitive Allele Specific PCR) assay was used for validating six marker loci linked to genes with resistance to Ug99. Of those, four co-segregated with the Sr25-pathotypes while the rest identified unknown resistance genes. With further investigation, these markers can be used for marker-assisted selection in breeding for Ug99 stem rust resistance in wheat. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
4697 |
| Topical term or geographic name as entry element |
Nucleotides |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Rusts |
| Miscellaneous information |
AGROVOC |
| Source of heading or term |
|
| 9 (RLIN) |
1251 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
1806 |
| Topical term or geographic name as entry element |
Spring wheat |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
2084 |
| Topical term or geographic name as entry element |
Chromosome mapping |
| Source of heading or term |
AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
1848 |
| Topical term or geographic name as entry element |
Genetic markers |
| Source of heading or term |
AGROVOC |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
3777 |
| Personal name |
Shiaoman Chao |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Singh, R.P. |
| Miscellaneous information |
Global Wheat Program |
| Field link and sequence number |
INT0610 |
| 9 (RLIN) |
825 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
2094 |
| Personal name |
Sorrells, M.E. |
| 773 0# - HOST ITEM ENTRY |
|---|
| Record control number |
u94957 |
| Place, publisher, and date of publication |
San Francisco, CA (USA) : Public Library of Science |
| Title |
PLoS One |
| Related parts |
v. 12, no. 2 : e0171963 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
|---|
| Link text |
Open Access through DSpace |
| Uniform Resource Identifier |
https://hdl.handle.net/10883/18608 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Article |
| Suppress in OPAC |
No |