An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design (Record no. 58263)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02689nab a22004217a 4500 |
| 001 - CONTROL NUMBER | |
| control field | 58263 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20191010225020.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 161010s2016 -uk|||p|op||| 00| 00eng d |
| 024 8# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | https://doi.org/10.1038/srep23890 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MX-TxCIM |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 4198 |
| Personal name | Yongping Zhao |
| 245 10 - TITLE STATEMENT | |
| Title | An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design |
| Medium | [Electronic Resource] |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | London : |
| Name of publisher, distributor, etc. | Nature Publishing Group, |
| Date of publication, distribution, etc. | 2016. |
| 500 ## - GENERAL NOTE | |
| General note | Peer review |
| 500 ## - GENERAL NOTE | |
| General note | Open Access |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Precision DNA/gene replacement is a promising genome-editing tool that is highly desirable for molecular engineering and breeding by design. Although the CRISPR/Cas9 system works well as a tool for gene knockout in plants, gene replacement has rarely been reported. Towards this end, we first designed a combinatory dual-sgRNA/Cas9 vector (construct #1) that successfully deleted miRNA gene regions (MIR169a and MIR827a). The deletions were confirmed by PCR and subsequent sequencing, yielding deletion efficiencies of 20% and 24% on MIR169a and MIR827a loci, respectively. We designed a second structure (construct #2) that contains sites homologous to Arabidopsis TERMINAL FLOWER 1 (TFL1) for homology-directed repair (HDR) with regions corresponding to the two sgRNAs on the modified construct #1. The two constructs were co-transformed into Arabidopsis plants to provide both targeted deletion and donor repair for targeted gene replacement by HDR. Four of 500 stably transformed T0 transgenic plants (0.8%) contained replaced fragments. The presence of the expected recombination sites was further confirmed by sequencing. Therefore, we successfully established a gene deletion/replacement system in stably transformed plants that can potentially be utilized to introduce genes of interest for targeted crop improvement. |
| 526 ## - STUDY PROGRAM INFORMATION NOTE | |
| Program name | Maize CRP |
| Maize Flagship Projects | FP2 - Novel tools, technologies and traits for improving genetic gains and breeding efficiency |
| -- | FP3 - Stress resilient and nutritious maize |
| 546 ## - LANGUAGE NOTE | |
| Language note | Text in English |
| 591 ## - CATALOGING NOTES | |
| Number of CIMMYT Informa | CIMMYT Informa: 1989 (April 20, 2017) |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 3563 |
| Topical term or geographic name as entry element | Genes |
| Source of heading or term | AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 4199 |
| Topical term or geographic name as entry element | Plants |
| Source of heading or term | AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 8831 |
| Topical term or geographic name as entry element | Genetic engineering |
| Miscellaneous information | AGROVOC |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 3444 |
| Topical term or geographic name as entry element | Molecular genetics |
| Source of heading or term | AGROVOC |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 4200 |
| Personal name | Congsheng Zhang |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 4201 |
| Personal name | Wenwen Liu |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 4202 |
| Personal name | Wei Gao |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 4203 |
| Personal name | Changlin Liu |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 4204 |
| Personal name | Gaoyuan Song |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 2733 |
| Personal name | Wen-Xue Li |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 4205 |
| Personal name | Long Mao |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 4206 |
| Personal name | Beijiu Chen |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 857 |
| Personal name | Yunbi Xu |
| Miscellaneous information | Global Maize Program |
| Field link and sequence number | INT2735 |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 4207 |
| Personal name | Xinhai Li |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 2730 |
| Personal name | Chuanxiao Xie |
| 773 0# - HOST ITEM ENTRY | |
| Record control number | a58025 |
| International Standard Serial Number | 2045-2322 |
| Place, publisher, and date of publication | London : Nature Publishing Group, 2011- |
| Title | Nature Scientific reports |
| Related parts | v. 6, no. 23890 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | http://hdl.handle.net/10883/18311 |
| Link text | Open Access through DSpace |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Article |
| Suppress in OPAC | No |
| Date last seen | Total Checkouts | Price effective from | Koha item type | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Withdrawn status | Home library | Current library | Date acquired |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 02/02/2017 | 02/02/2017 | Article | Not Lost | Dewey Decimal Classification | CIMMYT Staff Publications Collection | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Knowledge Center: John Woolston Library | 02/02/2017 |