MARC details
000 -LEADER |
fixed length control field |
03200nam a22004697a 4500 |
001 - CONTROL NUMBER |
control field |
G74651 |
003 - CONTROL NUMBER IDENTIFIER |
control field |
MX-TxCIM |
005 - DATE AND TIME OF LATEST TRANSACTION |
control field |
20240919021055.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
121211s ||||f| 0 p|p||0|| | |
020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
International Standard Book Number |
9974-7586-1-0 |
040 ## - CATALOGING SOURCE |
Original cataloging agency |
MX-TxCIM |
072 #0 - SUBJECT CATEGORY CODE |
Subject category code |
A50 |
072 #0 - SUBJECT CATEGORY CODE |
Subject category code |
F30 |
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
Classification number |
633.1153 |
Item number |
KOH |
100 1# - MAIN ENTRY--PERSONAL NAME |
Personal name |
Manifesto, M.M. |
Affiliation |
Application of Biotechnologies to Wheat Breeding. Proceedings of the Conference; La Estanzuela, Colonia, Uruguay; 19-20 Nov 1998 |
110 2# - MAIN ENTRY--CORPORATE NAME |
Corporate name or jurisdiction name as entry element |
Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico DF (Mexico) |
245 00 - TITLE STATEMENT |
Title |
Microsatellites in wheat: |
Remainder of title |
an useful tool for variety identification and breeding |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
Place of publication, distribution, etc. |
Montevideo (Uruguay) |
Name of publisher, distributor, etc. |
CIMMYT : |
Date of publication, distribution, etc. |
2000 |
300 ## - PHYSICAL DESCRIPTION |
Extent |
85-102 |
340 ## - PHYSICAL MEDIUM |
Material base and configuration |
Printed |
520 ## - SUMMARY, ETC. |
Summary, etc. |
Thousands of hypervariable regions called microsatellites or simple sequence repeats (SSR), present in plant and animal genomes, are characterized by two to four pairs of bases in tandem repeats. These loci can be amplified through Polymerase Chain Reaction (PCR) using specific primers (18-20 pb long), which recognize unique flanking sequences of the hypervariable regions. Abundant variability present within these loci together with loci specificity and its codominant nature makes this kind of marker a powerful tool for wheat research. In this study the microsatellite markers were evaluated as a potential tool for finger printing of the wheat cultivars and for the studies on genetic variability.||Microsatellites, located at different chromosomes, were used to analyze a set of 105 bread wheat varieties from Argentina. Variability for each microsatellite locus was measured using the Polymorphism Information Content (PIC). PIC values for the different loci varied between 0.40 and 0.84. These values were compared with those obtained through RFLP in the same set of varieties, that reached an average value of 0.46 for the HMW-GS loci. These results suggest that microsatellite markers were an appropriate tool to discriminate this set of varieties.||Closely related varieties, that present high similarity coefficients, can be discriminated using selected microsatellites. Finally, a subset of microsatellites was used to construct an Identity Matrix for the registered bread wheat cultivars that will allow an unequivocal identification of each variety. The chromosome location, range in size, number of alleles and PIC of each microsatellite loci were parameters used to build the Identity Matrix mentioned above. |
546 ## - LANGUAGE NOTE |
Language note |
English |
591 ## - CATALOGING NOTES |
Affiliation |
0105|AL-Wheat Program|AGRIS 0102 |
595 ## - COLLECTION |
Collection |
CIMMYT Publications Collection |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
DNA |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Genetic maps |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
9 (RLIN) |
1848 |
Topical term or geographic name as entry element |
Genetic markers |
Miscellaneous information |
AGROVOC |
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Genetic variation |
Miscellaneous information |
AGROVOC |
Source of heading or term |
|
9 (RLIN) |
1129 |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Identification |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Plant physiology |
9 (RLIN) |
1210 |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Selection |
653 0# - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
CIMMYT |
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Triticum |
Miscellaneous information |
AGROVOC |
Source of heading or term |
|
9 (RLIN) |
1295 |
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Plant breeding |
Miscellaneous information |
AGROVOC |
Source of heading or term |
|
9 (RLIN) |
1203 |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Dubcovsky, J., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Hopp, H.E., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Kohli, M.M.|Francis, M. |
Relator term |
eds. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Schlatter, A.R., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Suárez, E.Y., |
Relator term |
coaut. |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Koha item type |
Conference proceedings |