Microsatellites in wheat: (Record no. 5215)

000 -LEADER
fixed length control field 03166nam a22004697a 4500
001 - CONTROL NUMBER
control field 74651
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20190320161210.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 121211s ||||f| 0 p|p||0|| |
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 9974-7586-1-0
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
072 #0 - SUBJECT CATEGORY CODE
Subject category code A50
072 #0 - SUBJECT CATEGORY CODE
Subject category code F30
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 633.1153
Item number KOH
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Manifesto, M.M.
Affiliation Application of Biotechnologies to Wheat Breeding. Proceedings of the Conference; La Estanzuela, Colonia, Uruguay; 19-20 Nov 1998
110 2# - MAIN ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico DF (Mexico)
245 00 - TITLE STATEMENT
Title Microsatellites in wheat:
Remainder of title an useful tool for variety identification and breeding
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. Montevideo (Uruguay)
Name of publisher, distributor, etc. CIMMYT :
Date of publication, distribution, etc. 2000
300 ## - PHYSICAL DESCRIPTION
Extent 85-102
340 ## - PHYSICAL MEDIUM
Material base and configuration Printed
520 ## - SUMMARY, ETC.
Summary, etc. Thousands of hypervariable regions called microsatellites or simple sequence repeats (SSR), present in plant and animal genomes, are characterized by two to four pairs of bases in tandem repeats. These loci can be amplified through Polymerase Chain Reaction (PCR) using specific primers (18-20 pb long), which recognize unique flanking sequences of the hypervariable regions. Abundant variability present within these loci together with loci specificity and its codominant nature makes this kind of marker a powerful tool for wheat research. In this study the microsatellite markers were evaluated as a potential tool for finger printing of the wheat cultivars and for the studies on genetic variability.||Microsatellites, located at different chromosomes, were used to analyze a set of 105 bread wheat varieties from Argentina. Variability for each microsatellite locus was measured using the Polymorphism Information Content (PIC). PIC values for the different loci varied between 0.40 and 0.84. These values were compared with those obtained through RFLP in the same set of varieties, that reached an average value of 0.46 for the HMW-GS loci. These results suggest that microsatellite markers were an appropriate tool to discriminate this set of varieties.||Closely related varieties, that present high similarity coefficients, can be discriminated using selected microsatellites. Finally, a subset of microsatellites was used to construct an Identity Matrix for the registered bread wheat cultivars that will allow an unequivocal identification of each variety. The chromosome location, range in size, number of alleles and PIC of each microsatellite loci were parameters used to build the Identity Matrix mentioned above.
546 ## - LANGUAGE NOTE
Language note English
591 ## - CATALOGING NOTES
Affiliation 0105|AL-Wheat Program|AGRIS 0102
595 ## - COLLECTION
Collection CIMMYT Publications Collection
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element DNA
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Genetic maps
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 1848
Topical term or geographic name as entry element Genetic markers
Miscellaneous information AGROVOC
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Genetic variation
9 (RLIN) 1129
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Identification
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Plant physiology
9 (RLIN) 1210
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Selection
653 0# - INDEX TERM--UNCONTROLLED
Uncontrolled term CIMMYT
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 1295
Topical term or geographic name as entry element Triticum
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 1203
Topical term or geographic name as entry element Plant breeding
Miscellaneous information AGROVOC
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Dubcovsky, J.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Hopp, H.E.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Kohli, M.M.|Francis, M.
Relator term eds.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Schlatter, A.R.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Suárez, E.Y.,
Relator term coaut.
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Conference proceedings
Holdings
Withdrawn status Lost status Damaged status Not for loan Collection code Permanent Location Current Location Date acquired Full call number Barcode Date last seen Copy number Price effective from Koha item type
  Not Lost     CIMMYT Publications Collection CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 2015-02-10 633.1153 KOH D649448 2015-02-10 1 2015-02-10 Conference proceedings
baner

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2015. Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
Monday –Friday 9:00 am. 17:00 pm. If you have any question, please contact us at CIMMYT-Knowledge-Center@cgiar.org

Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT) © Copyright 2015. Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
Lunes –Viernes 9:00 am. 17:00 pm. Si tiene cualquier pregunta, contáctenos a CIMMYT-Knowledge-Center@cgiar.org