MARC details
| 000 -LEADER |
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| fixed length control field |
02736nam a22004457a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
G68374 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20211006075506.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
121211s ||||f| 0 p|p||0|| | |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
970-648-035-8 |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
MX-TxCIM |
| 072 #0 - SUBJECT CATEGORY CODE |
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| Subject category code |
F30 |
| 072 #0 - SUBJECT CATEGORY CODE |
|---|
| Subject category code |
H20 |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
632.4 |
| Item number |
GIN |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Murphy, N.E.A. |
| Affiliation |
Septoria and Stagonospora Diseases of Cereals: A Compilation of Global Research. Proceedings of International Workshop, 5. Mexico, D.F. (Mexico); 20-24 Sep 1999 |
| 110 2# - MAIN ENTRY--CORPORATE NAME |
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| Corporate name or jurisdiction name as entry element |
Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico DF (Mexico) |
| 245 00 - TITLE STATEMENT |
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| Title |
Identification of a molecular marker linked to Septoria nodorum blotch resistance in Triticum tauschii using F2 bulked segregant |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Place of publication, distribution, etc. |
Mexico, DF (Mexico) |
| Name of publisher, distributor, etc. |
CIMMYT : |
| Date of publication, distribution, etc. |
1999 |
| 340 ## - PHYSICAL MEDIUM |
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| Material base and configuration |
Printed |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
A search was conducted for a molecular marker linked to a gene for resistance to septoria nodorum blotch in the Triticum tauschii accession RL5271. DNA was extracted from leaves of F2 plants which were progeny tested to identify homozygous resistant and homozygous susceptible F2 plants. The DNA from the homozygous resistant plants was pooled together and the low copy sequences were enriched using renaturation kinetics and hydroxyapatite to remove the repetitive DNA sequences. The pooled DNA from the homozygous susceptible plants was treated in the same manner. The pooled DNA and the parental DNA were screened using RAPD primers. Two markers present in the pooled resistant DNA and in the parental DNA were identified and cloned. These markers were verified using RFLPs with the cloned marker as a probe. One of the probes did not produce any polymorphism as a RFLP, even when a number of different restriction enzymes were used. The second marker was polymorphic between the two parents when the DNA was restricted with HindIII and then MseI. In the 14 homozygous F2 plants tested, the marker was completely linked to the resistance gene. This marker may be valuable in introgressing the resistance gene from T. tauschii into a commercial bread wheat cultivar. |
| 546 ## - LANGUAGE NOTE |
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| Language note |
English |
| 591 ## - CATALOGING NOTES |
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| Affiliation |
9910|AGRIS 0001 |
| 595 ## - COLLECTION |
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| Collection |
CIMMYT Publications Collection |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
DNA |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Genetic resistance |
| 9 (RLIN) |
2277 |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Identification |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Molecular genetics |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Plant diseases |
| Miscellaneous information |
AGROVOC |
| Source of heading or term |
|
| 9 (RLIN) |
1206 |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Septoria |
| 653 0# - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
CIMMYT |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Triticum |
| Miscellaneous information |
AGROVOC |
| Source of heading or term |
|
| 9 (RLIN) |
1295 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
1980 |
| Personal name |
Appels, R. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ginkel, M. van|McNab, A.|Krupinsky, J. |
| Relator term |
eds. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Jones, M.G.K., |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Lagudah, E.S., |
| Relator term |
coaut. |
| 9 (RLIN) |
1697 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Loughman, R., |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Wilson, R.E., |
| Relator term |
coaut. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Conference proceedings |