MARC details
| 000 -LEADER |
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| fixed length control field |
02676nam a22004097a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
G66303 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20211006073315.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
121211s ||||f| 0 p|p||0|| | |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
970-648-001-3 |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
MX-TxCIM |
| 072 #0 - SUBJECT CATEGORY CODE |
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| Subject category code |
F30 |
| 072 #0 - SUBJECT CATEGORY CODE |
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| Subject category code |
H20 |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
633.1194 |
| Item number |
DUV |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Emami, K. |
| Affiliation |
Helminthosporium Blights of Wheat: Spot Blotch and Tan Spot. Proceedings of an International Workshop; El Batan, Texcoco (Mexico); 9 - 14 Feb 1997 |
| 110 2# - MAIN ENTRY--CORPORATE NAME |
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| Corporate name or jurisdiction name as entry element |
Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico DF (Mexico) |
| 245 00 - TITLE STATEMENT |
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| Title |
A xylanase gene from Cochliobolus sativus |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Place of publication, distribution, etc. |
Mexico, DF (Mexico) |
| Name of publisher, distributor, etc. |
CIMMYT|UCL|BADC : |
| Date of publication, distribution, etc. |
1998 |
| 340 ## - PHYSICAL MEDIUM |
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| Material base and configuration |
Printed |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Cell-wall-degrading enzymes are expected to be important inpathogenesis by Cochliobolus sativus (Bipolaris sorokiniana). The xylanase activity of an isolate from barley was assayed in five different culture media. The highest activity was in Fries' medium containing 2% oat spelt xylan, 3% cellulose, and 4% peptone. To investigate genes coding for cell-wall-degrading enzymes in C. sativus, total DNA was digested with restriction enzymes and probed with C. carbonum XYL1 and PGN1 cDNAs, coding for a xylanase and an endo- polygalacturonase. The hybridization patterns indicate that C. sativus has genes resembling both. A cDNA library was constructed from mRNA of C. sativus grown on xylanase-inducing medium, and screened with C. carbonum XYL1 cDNA. All xylanase-encoding clones identified in this way were more similar to a second C. carbonum xylanase gene, XYL2, than to XYL1. Part of the C. sativus xylanase gene corresponding to the clones was amplified by the polymerase chain reaction (PCR), and found to have two introns like C. carbonum XYL2. Amplification with reduced stringency gave a second PCR product; DNA sequencing showed that it is derived from the C. sativus homologue of the C. carbonum XYL1 gene, with a single intron. The 5¢ and 3¢ flanking regions of the C. sativus XYL2 homologue were amplified by inverse PCR. The resemblance between the C. sativus and C. carbonum XYL2 sequences extends to the ends of the reported C. carbonum sequence. |
| 546 ## - LANGUAGE NOTE |
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| Language note |
English |
| 591 ## - CATALOGING NOTES |
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| Affiliation |
9806|AGRIS 9802 |
| 595 ## - COLLECTION |
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| Collection |
CIMMYT Publications Collection |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Barley |
| 9 (RLIN) |
1018 |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Bipolaris |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Cochliobolus |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
DNA |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Enzymes |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Pathogens |
| 9 (RLIN) |
1197 |
| 650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Plant diseases |
| Miscellaneous information |
AGROVOC |
| Source of heading or term |
|
| 9 (RLIN) |
1206 |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Testing |
| 653 0# - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
CIMMYT |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Duveiller, E.|Dubin, H.J.|Reeves, J.|McNab, A. |
| Relator term |
eds. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Hack, E., |
| Relator term |
coaut. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Koha item type |
Conference proceedings |