Knowledge Center Catalog

Enhancing laccase production by a newly-isolated strain of Pycnoporus sanguineus with high potential for dye decolouration (Record no. 30950)

MARC details
000 -LEADER
fixed length control field 02632nab a22003497a 4500
001 - CONTROL NUMBER
control field G99726
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20171220113637.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 121211b |||p||p||||||| |z||| |
024 8# - OTHER STANDARD IDENTIFIER
Standard number or code https://doi.org/10.1039/C4RA06039C
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title En
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Gioia, L.
245 00 - TITLE STATEMENT
Title Enhancing laccase production by a newly-isolated strain of Pycnoporus sanguineus with high potential for dye decolouration
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Date of publication, distribution, etc. 2014
500 ## - GENERAL NOTE
General note Peer-review: No - Open Access: Yes |http://www.rsc.org/publishing/journals/ra/about.asp
500 ## - GENERAL NOTE
General note Reference only
520 ## - SUMMARY, ETC.
Summary, etc. Response surface methodology (RSM) was applied to optimise laccase production by a new strain of Pycnoporus sanguineus, isolated from the Uruguayan forests, grown on bark shavings under semi-solid-state fermentation conditions. Enhancement of laccase production was studied, with a full 24 factorial design with two replicates and four central points. Operating under the optimal conditions obtained (pH 3.0, yeast extract 10 g L−1, glucose 5 g L−1 and bark shavings 3 g), a laccase activity of nearly 3000 U L−1 was achieved. Subsequently, the effect of different potential inducers on laccase production (Cu+2, Mn+2 and lignin) was tested using the one-factor-at-a-time approach. It was found that the addition of 0.5 mM Cu+2 into the cultures considerably increased laccase production and, thus, maximum activity values of about 16 000 U L−1 were achieved. Further, the optimised culture broth (containing mainly laccase) showed high capacity to decolourise different recalcitrant synthetic dyes such as Acid Red 88 (AR88), Reactive Black 5 (RB5) and Lanaset Grey G (LG). After selecting enzyme concentration and time of reaction, almost 70% of AR88 could be decolourised in the absence of redox mediators. However, mediators were necessary to bleach RB5 and LG. Methyl syringate was the most effective mediator for AR88 (reaching a decolouration degree over 90%) and RB5 (nearly 70%). Around 80% of LG could be decolourised in the presence of violuric acid. These promising results make this enzyme a very useful tool for developing wastewater treatments.
536 ## - FUNDING INFORMATION NOTE
Text of note Genetic Resources Program
546 ## - LANGUAGE NOTE
Language note English
591 ## - CATALOGING NOTES
Affiliation CIMMYT Informa 2015
594 ## - STAFFID
StaffID INT3239
595 ## - COLLECTION
Collection CIMMYT Staff Publications Collection
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Burgue±o, J.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Manta, C.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Menendeza, P.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Ovsejevi, K.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Rodriguez-Couto, S.,
Relator term coaut.
773 0# - HOST ITEM ENTRY
Title RSC Advances
Related parts v. 64, p. 34096-34103
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Holdings
Date last seen Total Checkouts Price effective from Koha item type Lost status Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
06/29/2017   06/29/2017 Article Not Lost     CIMMYT Staff Publications Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 06/29/2017

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