A novel method for determination of the 15N isotopic composition of Rubisco in wheat plants exposed to elevated atmospheric carbon dioxide (Record no. 30903)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02109nab a22003137a 4500 |
| 001 - CONTROL NUMBER | |
| control field | G99611 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20210505163929.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 121211b |||p||p||||||| |z||| | |
| 022 0# - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 0031-9317 |
| 024 8# - OTHER STANDARD IDENTIFIER | |
| Standard number or code | https://doi.org/10.1111/ppl.12294 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MX-TxCIM |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | En |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Aranjuelo, I. |
| 245 00 - TITLE STATEMENT | |
| Title | A novel method for determination of the 15N isotopic composition of Rubisco in wheat plants exposed to elevated atmospheric carbon dioxide |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Date of publication, distribution, etc. | 2015 |
| 500 ## - GENERAL NOTE | |
| General note | Peer-review: Yes - Yes |http://ip-science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&Full=Physiologia%20Plantarum |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Although ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is mostly known as a key enzyme involved in CO2 assimilation during the Calvin cycle, comparatively little is known about its role as a pool of nitrogen storage in leaves. For this purpose, we developed a protocol to purify Rubisco that enables later analysis of its 15N isotope composition (δ15N) at the natural abundance and 15N-labeled plants. In order to test the utility of this protocol, durum wheat (Triticum durum var. Sula) exposed to an elevated CO2 concentration (700 vs 400 ?mol mol−1) was labeled with K15NO3 (enriched at 2 atom %) during the ear development period. The developed protocol proves to be selective, simple, cost effective and reproducible. The study reveals that 15N labeling was different in total organic matter, total soluble protein and the Rubisco fraction. The obtained data suggest that photosynthetic acclimation in wheat is caused by Rubisco depletion. This depletion may be linked to preferential nitrogen remobilization from Rubisco toward grain filling. |
| 536 ## - FUNDING INFORMATION NOTE | |
| Text of note | Global Wheat Program |
| 546 ## - LANGUAGE NOTE | |
| Language note | English |
| 591 ## - CATALOGING NOTES | |
| Affiliation | Wiley|CIMMYT Informa 2015 |
| 594 ## - STAFFID | |
| StaffID | INT3193 |
| 595 ## - COLLECTION | |
| Collection | CIMMYT Staff Publications Collection |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Avice, J.C., |
| Relator term | coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bourguignon, J., |
| Relator term | coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 899 |
| Personal name | Molero, G. |
| Miscellaneous information | Formerly Global Wheat Program |
| Field link and sequence number | INT3193 |
| 773 0# - HOST ITEM ENTRY | |
| Title | Physiologia Plantarum |
| Related parts | v. 153, no. 2, p. 195-203 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Article |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Date last seen | Total Checkouts | Price effective from | Koha item type | Lost status | Damaged status | Not for loan | Collection code | Withdrawn status | Home library | Current library | Date acquired |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 07/03/2017 | 07/03/2017 | Article | Not Lost | CIMMYT Staff Publications Collection | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Knowledge Center: John Woolston Library | 07/03/2017 |