Knowledge Center Catalog

On the relationship between C and N fixation and amino acid synthesis in nodulated alfalfa (Medicago sativa) (Record no. 30354)

MARC details
000 -LEADER
fixed length control field 03187nab a22004097a 4500
001 - CONTROL NUMBER
control field G98458
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20210505163929.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 121211b |||p||p||||||| |z||| |
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 1445-4416 (Revista en electrónico)
022 0# - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 1445-4408
024 8# - OTHER STANDARD IDENTIFIER
Standard number or code https://doi.org/10.1071/FP13189
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) CIS-7431
100 1# - MAIN ENTRY--PERSONAL NAME
9 (RLIN) 899
Personal name Molero, G.
Miscellaneous information Formerly Global Wheat Program
Field link and sequence number INT3193
245 00 - TITLE STATEMENT
Title On the relationship between C and N fixation and amino acid synthesis in nodulated alfalfa (Medicago sativa)
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Date of publication, distribution, etc. 2014
500 ## - GENERAL NOTE
General note Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1445-4408
520 ## - SUMMARY, ETC.
Summary, etc. Legumes such as alfalfa (Medicago sativa L.) are vital N2-fixing crops accounting for a global N2 fixation of ~35 Mt N year. Although enzymatic and molecular mechanisms of nodule N2 fixation are now well documented, some uncertainty remains as to whether N2 fixation is strictly coupled with photosynthetic carbon fixation. That is, the metabolic origin and redistribution of carbon skeletons used to incorporate nitrogen are still relatively undefined. Here, we conducted isotopic labelling with both 15N2 and 13C-depleted CO2 on alfalfa plants grown under controlled conditions and took advantage of isotope ratio mass spectrometry to investigate the relationship between carbon and nitrogen turn-over in respired CO2, total organic matter and amino acids. Our results indicate that CO2 evolved by respiration had an isotopic composition similar to that in organic matter regardless of the organ considered, suggesting that the turn-over of respiratory pools strictly followed photosynthetic input. However, carbon turn-over was nearly three times greater than N turn-over in total organic matter, suggesting that new organic material synthesised was less N-rich than pre-existing organic material (due to progressive nitrogen elemental dilution) or that N remobilisation occurred to sustain growth. This pattern was not consistent with the total commitment into free amino acids where the input of new C and N appeared to be stoichiometric. The labelling pattern in Asn was complex, with contrasted C and N commitments in different organs, suggesting that neosynthesis and redistribution of new Asn molecules required metabolic remobilisation. We conclude that the production of new organic material during alfalfa growth depends on both C and N remobilisation in different organs. At the plant level, this remobilisation is complicated by allocation and metabolism in the different organs.
536 ## - FUNDING INFORMATION NOTE
Text of note Global Wheat Program
546 ## - LANGUAGE NOTE
Language note English
591 ## - CATALOGING NOTES
Affiliation CSIRO|CIMMYT Informa No. 1907
594 ## - STAFFID
StaffID INT3193
595 ## - COLLECTION
Collection CIMMYT Staff Publications Collection
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element carbon exchange
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element carbon isotopes
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element nitrogen 15 isotope
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Nitrogen fixation
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 1913
Topical term or geographic name as entry element Photosynthesis
Miscellaneous information AGROVOC
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Respiration
700 1# - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 1436
Personal name Araus, J.L.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Nogues, S.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Tcherkez, G.,
Relator term coaut.
773 0# - HOST ITEM ENTRY
Title Functional Plant Biology
Related parts v. 41, no. 4, p. 331-341
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Holdings
Date last seen Total Checkouts Full call number Price effective from Koha item type Lost status Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
07/03/2017   CIS-7431 07/03/2017 Article Not Lost     CIMMYT Staff Publications Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 07/03/2017

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