MARC details
000 -LEADER |
fixed length control field |
03187nab a22004097a 4500 |
001 - CONTROL NUMBER |
control field |
G98458 |
003 - CONTROL NUMBER IDENTIFIER |
control field |
MX-TxCIM |
005 - DATE AND TIME OF LATEST TRANSACTION |
control field |
20210505163929.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
121211b |||p||p||||||| |z||| | |
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
International Standard Serial Number |
1445-4416 (Revista en electrónico) |
022 0# - INTERNATIONAL STANDARD SERIAL NUMBER |
International Standard Serial Number |
1445-4408 |
024 8# - OTHER STANDARD IDENTIFIER |
Standard number or code |
https://doi.org/10.1071/FP13189 |
040 ## - CATALOGING SOURCE |
Original cataloging agency |
MX-TxCIM |
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
CIS-7431 |
100 1# - MAIN ENTRY--PERSONAL NAME |
9 (RLIN) |
899 |
Personal name |
Molero, G. |
Miscellaneous information |
Formerly Global Wheat Program |
Field link and sequence number |
INT3193 |
245 00 - TITLE STATEMENT |
Title |
On the relationship between C and N fixation and amino acid synthesis in nodulated alfalfa (Medicago sativa) |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
Date of publication, distribution, etc. |
2014 |
500 ## - GENERAL NOTE |
General note |
Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1445-4408 |
520 ## - SUMMARY, ETC. |
Summary, etc. |
Legumes such as alfalfa (Medicago sativa L.) are vital N2-fixing crops accounting for a global N2 fixation of ~35 Mt N year. Although enzymatic and molecular mechanisms of nodule N2 fixation are now well documented, some uncertainty remains as to whether N2 fixation is strictly coupled with photosynthetic carbon fixation. That is, the metabolic origin and redistribution of carbon skeletons used to incorporate nitrogen are still relatively undefined. Here, we conducted isotopic labelling with both 15N2 and 13C-depleted CO2 on alfalfa plants grown under controlled conditions and took advantage of isotope ratio mass spectrometry to investigate the relationship between carbon and nitrogen turn-over in respired CO2, total organic matter and amino acids. Our results indicate that CO2 evolved by respiration had an isotopic composition similar to that in organic matter regardless of the organ considered, suggesting that the turn-over of respiratory pools strictly followed photosynthetic input. However, carbon turn-over was nearly three times greater than N turn-over in total organic matter, suggesting that new organic material synthesised was less N-rich than pre-existing organic material (due to progressive nitrogen elemental dilution) or that N remobilisation occurred to sustain growth. This pattern was not consistent with the total commitment into free amino acids where the input of new C and N appeared to be stoichiometric. The labelling pattern in Asn was complex, with contrasted C and N commitments in different organs, suggesting that neosynthesis and redistribution of new Asn molecules required metabolic remobilisation. We conclude that the production of new organic material during alfalfa growth depends on both C and N remobilisation in different organs. At the plant level, this remobilisation is complicated by allocation and metabolism in the different organs. |
536 ## - FUNDING INFORMATION NOTE |
Text of note |
Global Wheat Program |
546 ## - LANGUAGE NOTE |
Language note |
English |
591 ## - CATALOGING NOTES |
Affiliation |
CSIRO|CIMMYT Informa No. 1907 |
594 ## - STAFFID |
StaffID |
INT3193 |
595 ## - COLLECTION |
Collection |
CIMMYT Staff Publications Collection |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
carbon exchange |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
carbon isotopes |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
nitrogen 15 isotope |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Nitrogen fixation |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
9 (RLIN) |
1913 |
Topical term or geographic name as entry element |
Photosynthesis |
Miscellaneous information |
AGROVOC |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Respiration |
700 1# - ADDED ENTRY--PERSONAL NAME |
9 (RLIN) |
1436 |
Personal name |
Araus, J.L. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Nogues, S., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Tcherkez, G., |
Relator term |
coaut. |
773 0# - HOST ITEM ENTRY |
Title |
Functional Plant Biology |
Related parts |
v. 41, no. 4, p. 331-341 |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Koha item type |
Article |