MARC details
000 -LEADER |
fixed length control field |
03706nab a22004337a 4500 |
001 - CONTROL NUMBER |
control field |
G98291 |
003 - CONTROL NUMBER IDENTIFIER |
control field |
MX-TxCIM |
005 - DATE AND TIME OF LATEST TRANSACTION |
control field |
20171220113619.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
121211b |||p||p||||||| |z||| | |
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
International Standard Serial Number |
No (Revista en electrónico) |
022 0# - INTERNATIONAL STANDARD SERIAL NUMBER |
International Standard Serial Number |
1471-2229 |
024 8# - OTHER STANDARD IDENTIFIER |
Standard number or code |
https://doi.org/10.1186/1471-2229-12-9 |
040 ## - CATALOGING SOURCE |
Original cataloging agency |
MX-TxCIM |
100 1# - MAIN ENTRY--PERSONAL NAME |
9 (RLIN) |
922 |
Personal name |
Sehgal, D. |
Miscellaneous information |
Global Wheat Program |
Field link and sequence number |
INT3332 |
245 00 - TITLE STATEMENT |
Title |
Integration of gene-based markers in a pearl millet genetic map for identification of candidate genes underlying drought tolerance quantitative trait loci |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
Date of publication, distribution, etc. |
2012 |
500 ## - GENERAL NOTE |
General note |
Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1471-2229 |
520 ## - SUMMARY, ETC. |
Summary, etc. |
Background: Identification of genes underlying drought tolerance (DT) quantitative trait loci (QTLs) will facilitate understanding of molecular mechanisms of drought tolerance, and also will accelerate genetic improvement of pearl millet through marker-assisted selection. We report a map based on genes with assigned functional roles in plant adaptation to drought and other abiotic stresses and demonstrate its use in identifying candidate genes underlying a major DT-QTL. Results: Seventy five single nucleotide polymorphism (SNP) and conserved intron spanning primer (CISP) markers were developed from available expressed sequence tags (ESTs) using four genotypes, H 77/833-2, PRLT 2/89-33, ICMR 01029 and ICMR 01004, representing parents of two mapping populations. A total of 228 SNPs were obtained from 30.5 kb sequenced region resulting in a SNP frequency of 1/134 bp. The positions of major pearl millet linkage group (LG) 2 DT-QTLs (reported from crosses H 77/833-2 × PRLT 2/89-33 and 841B × 863B) were added to the present consensus function map which identified 18 genes, coding for PSI reaction center subunit III, PHYC, actin, alanine glyoxylate aminotransferase, uridylate kinase, acyl-CoA oxidase, dipeptidyl peptidase IV, MADS-box, serine/threonine protein kinase, ubiquitin conjugating enzyme, zinc finger C- × 8-C × 5-C × 3-H type, Hd3, acetyl CoA carboxylase, chlorophyll a/b binding protein, photolyase, protein phosphatase1 regulatory subunit SDS22 and two hypothetical proteins, co-mapping in this DT-QTL interval. Many of these candidate genes were found to have significant association with QTLs of grain yield, flowering time and leaf rolling under drought stress conditions. Conclusions: We have exploited available pearl millet EST sequences to generate a mapped resource of seventy five new gene-based markers for pearl millet and demonstrated its use in identifying candidate genes underlying a major DT-QTL in this species. The reported gene-based markers represent an important resource for identification of candidate genes for other mapped abiotic stress QTLs in pearl millet. They also provide a resource for initiating association studies using candidate genes and also for comparing the structure and function of distantly related plant genomes such as other Poaceae members. |
536 ## - FUNDING INFORMATION NOTE |
Text of note |
Global Wheat Program |
546 ## - LANGUAGE NOTE |
Language note |
English |
591 ## - CATALOGING NOTES |
Affiliation |
No CIMMYT affiliation |
594 ## - STAFFID |
StaffID |
INT3332 |
595 ## - COLLECTION |
Collection |
Reprints Collection |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Candidate genes |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
CISP |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Drought tolerance QTLs |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
EST-SSR |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Pearl millet |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
SNP |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Armstead, I.P., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Hash, C.T., |
Relator term |
coaut. |
9 (RLIN) |
2442 |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Rajaram, V., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Singh Yadav, R., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Vadez, V., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Yash Pal Yadav, |
Relator term |
coaut. |
773 0# - HOST ITEM ENTRY |
Title |
BMC Plant Biology |
Related parts |
v. 12, no. 9, p. 13 p. |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Koha item type |
Article |