MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03033nab a22004577a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
G98112 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20230417202533.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
210709s2013 ne |||p|op||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
1568-5411 (Online) |
| 022 0# - INTERNATIONAL STANDARD SERIAL NUMBER |
|---|
| International Standard Serial Number |
1388-5545 |
| 024 8# - OTHER STANDARD IDENTIFIER |
|---|
| Standard number or code |
https://doi.org/10.1163/15685411-00002713 |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
MX-TxCIM |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
CIS-7261 |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
404 |
| Personal name |
Toumi, F. |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Development of a species-specific PCR to detect the cereal cyst nematode, Heterodera latipons |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc. |
Leiden (Netherlands) : |
| Name of publisher, distributor, etc. |
Brill, |
| Date of publication, distribution, etc. |
2013. |
| 500 ## - GENERAL NOTE |
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| General note |
Peer review |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=1388-5545 |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Several Heterodera species can reduce the yield of wheat and barley, among which H. avenae, H. filipjevi and H. latipons are economically the most important. Their identification, based on morphological characteristics, is not straightforward but can be made easier using molecular techniques. In this study, we developed species-specific primers for the detection of H. latipons. The actin gene of eight Heterodera species was partially sequenced and, after purifying and sequencing the PCR products, all sequences were aligned to find unique sites. The alignment showed moderate to very high similarities between the species. However, a small fragment of the actin gene was suitable for the construction of a potentially useful species-specific primer for H. latipons. The optimised PCR was subsequently tested with several populations of 14 Heterodera species and a single population of Punctodera punctata. Heterodera latipons was represented by 16 populations originating from six different countries. The primer set (Hlat-act), designed using AlleleID 7.73, was shown to be very specific. To test its sensitivity further, the PCR was conducted on DNA extracted from five second-stage juveniles (J2) of H. latipons mixed with five or 100 J2 belonging to H. avenae. The PCR was able to detect up to 1:10 dilution of the DNA obtained from five J2. The results showed that a specific and sensitive H. latipons species-specific PCR was constructed. |
| 536 ## - FUNDING INFORMATION NOTE |
|---|
| Text of note |
Global Wheat Program |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English |
| 591 ## - CATALOGING NOTES |
|---|
| Affiliation |
CIMMYT Informa No. 1860 |
| 594 ## - STAFFID |
|---|
| StaffID |
INT2918|INT2410 |
| 595 ## - COLLECTION |
|---|
| Collection |
CIMMYT Staff Publications Collection |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Actin |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
30707 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Genes |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
3563 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Diagnosis |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
5807 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Nucleotide sequence |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1937 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Genetic markers |
| Source of heading or term |
AGROVOC |
| 9 (RLIN) |
1848 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
363 |
| Personal name |
Waeyenberge, L. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
358 |
| Personal name |
Viaene, N. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Dababat, A.A. |
| 9 (RLIN) |
874 |
| Field link and sequence number |
INT2918 |
| Miscellaneous information |
Global Wheat Program |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Nicol, J.M. |
| 9 (RLIN) |
228 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ogbonnaya, F.C. |
| 9 (RLIN) |
237 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Moens, M. |
| 9 (RLIN) |
212 |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Nematology |
| Related parts |
v. 15, no. 6, p. 709-717 |
| Place, publisher, and date of publication |
Leiden (Netherlands) : Brill, 2013. |
| Record control number |
57516 |
| International Standard Serial Number |
1568-5411 |
| 856 4# - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
https://hdl.handle.net/20.500.12665/596 |
| Link text |
Access only for CIMMYT Staff |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Suppress in OPAC |
No |