Knowledge Center Catalog

The hrp genes cluster in erwinia pyrifoliae and determination of hr active domain in hrpnep protein (Record no. 29904)

MARC details
000 -LEADER
fixed length control field 02868nab a22004337a 4500
001 - CONTROL NUMBER
control field G97742
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20190927225229.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 121211b |||p||p||||||| |z||| |
022 0# - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 0567-7572
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title En
100 1# - MAIN ENTRY--PERSONAL NAME
9 (RLIN) 864
Personal name Shrestha, R.
Miscellaneous information Genetic Resources Program
Field link and sequence number INT2832
245 00 - TITLE STATEMENT
Title The hrp genes cluster in erwinia pyrifoliae and determination of hr active domain in hrpnep protein
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Date of publication, distribution, etc. 2008
500 ## - GENERAL NOTE
General note Reference only
520 ## - SUMMARY, ETC.
Summary, etc. Previously, the disease-specific (dsp) region and the hypersensitive response and pathogenicity (hrp) genes including the hrpW, hrpNEp and hrpC operons were sequenced in Erwinia pyrifoliae WT3. In this study, we therefore, determined the remaining hrp genes including the hrpC, hrpA, hrpS, hrpXY, hrpL and hrpJ operons and sequenced completely. The genetic organization of the hrp/hrc genes and their orientation for the transcriptions were also similar to and collinear with those of E. amylovora, showing ¡Ý80% homologies. However, ORFU1 and ORFU2 of unknown functions, present between the hrpA and hrpS operons of E. amylovora, were absent in E. pyrifoliae. To determine the HR active domains, several proteins were prepared from truncated fragments of the N-terminal and the C-terminal regions of HrpNEp of E. pyrifoliae. The proteins prepared from the N-terminal region elicited HR but not from those of the C-terminal region indicating that HR active domains are located in only N-terminal region of the HrpNEp protein. Two synthetic oligopeptides produced HR on tobacco confirming presence of two HR active domains in the HrpNEp. The HR positive N-terminal fragment (HN¦¤C187) was further narrowed down by deleting C-terminal amino acids and internal amino acids to investigate whether amino acid insertion region have role in faster and stronger HR activity in HrpNEp than HrpNEa. Deletion of third amino acid insertion region showed reduction in HR activation when compared to wild type. However, deletion of other amino acid insertion regions did not cause any change in HR elicitation speculating the importance of third amino acid insertion region in HrpNEp for causing faster HR activity.
536 ## - FUNDING INFORMATION NOTE
Text of note Genetic Resources Program
546 ## - LANGUAGE NOTE
Language note English
591 ## - CATALOGING NOTES
Affiliation No CIMMYT affiliation
594 ## - STAFFID
StaffID INT2832
595 ## - COLLECTION
Collection Reprints Collection
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element dsp/hrp
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element HR
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Mutagenesis
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Pyrus pyrifolia
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Shoot Blight
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Cho, J.M.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Cho, S.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Hong, S.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Hur, J.H.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Hwang, I.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Kim, W.-S.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Lee, S.H.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Lim, C.K.,
Relator term coaut.
773 0# - HOST ITEM ENTRY
Title Acta Horticulturae
Related parts v. 793, p. 221-230
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Holdings
Date last seen Total Checkouts Price effective from Koha item type Lost status Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
06/29/2017   06/29/2017 Article Not Lost     Reprints Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 06/29/2017

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