MARC details
000 -LEADER |
fixed length control field |
03389nab a22005657a 4500 |
001 - CONTROL NUMBER |
control field |
G97705 |
003 - CONTROL NUMBER IDENTIFIER |
control field |
MX-TxCIM |
005 - DATE AND TIME OF LATEST TRANSACTION |
control field |
20191008225029.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
121211b |||p||p||||||| |z||| | |
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
International Standard Serial Number |
1573-5028 (Revista en electrónico) |
022 0# - INTERNATIONAL STANDARD SERIAL NUMBER |
International Standard Serial Number |
0167-4412 |
024 8# - OTHER STANDARD IDENTIFIER |
Standard number or code |
https://doi.org/10.1007/s11103-008-9303-y |
040 ## - CATALOGING SOURCE |
Original cataloging agency |
MX-TxCIM |
041 0# - LANGUAGE CODE |
Language code of text/sound track or separate title |
En |
100 1# - MAIN ENTRY--PERSONAL NAME |
Personal name |
Bernard, S.M. |
245 00 - TITLE STATEMENT |
Title |
Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.) |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
Date of publication, distribution, etc. |
2008 |
500 ## - GENERAL NOTE |
General note |
Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0167-4412 |
520 ## - SUMMARY, ETC. |
Summary, etc. |
We present the first cloning and study of glutamine synthetase (GS) genes in wheat (Triticum aestivum L.). Based on sequence analysis, phylogenetic studies and mapping data, ten GS sequences were classified into four sub-families: GS2 (a, b and c), GS1 (a, b and c), GSr (1 and 2) and GSe (1 and 2). Phylogenetic analysis showed that the wheat GS sub-families together with the GS genes from other monocotyledonous species form four distinct clades. Immunolocalisation studies in leaves, stems and rachis in plants at flowering showed GS protein to be present in parenchyma, phloem companion and perifascicular sheath cells. In situ localisation confirmed that GS1 transcripts were present in the perifascicular sheath cells whilst those for GSr were confined to the vascular cells. Studies of the expression and protein profiles showed that all GS sub-families were differentially expressed in the leaves, peduncle, glumes and roots. Expression of GS genes in leaves was developmentally regulated, with both GS2 and GS1 assimilating or recycling ammonia in leaves during the period of grain development and filling. During leaf senescence the cytosolic isozymes, GS1 and GSr, were the predominant forms, suggesting major roles in assimilating ammonia during the critical phases of remobilisation of nitrogen to the grain. A preliminary analysis of three different wheat genotypes showed that the ratio of leaf GS2 protein to GS1 protein was variable. Use of this genetic variation should inform future efforts to modulate this enzyme for pre-breeding efforts to improve nitrogen use in wheat. |
536 ## - FUNDING INFORMATION NOTE |
Text of note |
Global Wheat Program |
546 ## - LANGUAGE NOTE |
Language note |
English |
591 ## - CATALOGING NOTES |
Affiliation |
No CIMMYT affiliation |
594 ## - STAFFID |
StaffID |
INT2835 |
595 ## - COLLECTION |
Collection |
Reprints Collection |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Gene expression |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Glutamine synthetase |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Immunolocalisation |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
In situ hybridisation |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Nitrogen |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Senescence |
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Wheat |
700 1# - ADDED ENTRY--PERSONAL NAME |
9 (RLIN) |
1436 |
Personal name |
Araus, J.L. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Baudo, M., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Blom Moller, A.L., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Dionisio, G., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Dubois, F., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Forde, B.G., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Foyer, C.H., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Habash, D.Z., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Hichey, T., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Hirel, B., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Jahn, T.P., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
9 (RLIN) |
1912 |
Personal name |
Parry, M.A.J. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Schjoerring, J.K., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Terce-Laforgue, T., |
Relator term |
coaut. |
700 1# - ADDED ENTRY--PERSONAL NAME |
9 (RLIN) |
865 |
Personal name |
Lopes, M.S. |
Miscellaneous information |
Global Wheat Program |
Field link and sequence number |
INT2835 |
773 0# - HOST ITEM ENTRY |
Title |
Plant Molecular Biology |
Related parts |
v. 67, no. 1-2, p. 89-105 |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Koha item type |
Article |