Knowledge Center Catalog

Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.) (Record no. 29877)

MARC details
000 -LEADER
fixed length control field 03389nab a22005657a 4500
001 - CONTROL NUMBER
control field G97705
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20191008225029.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 121211b |||p||p||||||| |z||| |
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 1573-5028 (Revista en electrónico)
022 0# - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 0167-4412
024 8# - OTHER STANDARD IDENTIFIER
Standard number or code https://doi.org/10.1007/s11103-008-9303-y
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title En
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Bernard, S.M.
245 00 - TITLE STATEMENT
Title Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.)
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Date of publication, distribution, etc. 2008
500 ## - GENERAL NOTE
General note Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0167-4412
520 ## - SUMMARY, ETC.
Summary, etc. We present the first cloning and study of glutamine synthetase (GS) genes in wheat (Triticum aestivum L.). Based on sequence analysis, phylogenetic studies and mapping data, ten GS sequences were classified into four sub-families: GS2 (a, b and c), GS1 (a, b and c), GSr (1 and 2) and GSe (1 and 2). Phylogenetic analysis showed that the wheat GS sub-families together with the GS genes from other monocotyledonous species form four distinct clades. Immunolocalisation studies in leaves, stems and rachis in plants at flowering showed GS protein to be present in parenchyma, phloem companion and perifascicular sheath cells. In situ localisation confirmed that GS1 transcripts were present in the perifascicular sheath cells whilst those for GSr were confined to the vascular cells. Studies of the expression and protein profiles showed that all GS sub-families were differentially expressed in the leaves, peduncle, glumes and roots. Expression of GS genes in leaves was developmentally regulated, with both GS2 and GS1 assimilating or recycling ammonia in leaves during the period of grain development and filling. During leaf senescence the cytosolic isozymes, GS1 and GSr, were the predominant forms, suggesting major roles in assimilating ammonia during the critical phases of remobilisation of nitrogen to the grain. A preliminary analysis of three different wheat genotypes showed that the ratio of leaf GS2 protein to GS1 protein was variable. Use of this genetic variation should inform future efforts to modulate this enzyme for pre-breeding efforts to improve nitrogen use in wheat.
536 ## - FUNDING INFORMATION NOTE
Text of note Global Wheat Program
546 ## - LANGUAGE NOTE
Language note English
591 ## - CATALOGING NOTES
Affiliation No CIMMYT affiliation
594 ## - STAFFID
StaffID INT2835
595 ## - COLLECTION
Collection Reprints Collection
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Gene expression
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Glutamine synthetase
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Immunolocalisation
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element In situ hybridisation
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Nitrogen
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Senescence
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Wheat
700 1# - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 1436
Personal name Araus, J.L.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Baudo, M.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Blom Moller, A.L.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Dionisio, G.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Dubois, F.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Forde, B.G.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Foyer, C.H.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Habash, D.Z.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Hichey, T.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Hirel, B.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Jahn, T.P.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 1912
Personal name Parry, M.A.J.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Schjoerring, J.K.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Terce-Laforgue, T.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 865
Personal name Lopes, M.S.
Miscellaneous information Global Wheat Program
Field link and sequence number INT2835
773 0# - HOST ITEM ENTRY
Title Plant Molecular Biology
Related parts v. 67, no. 1-2, p. 89-105
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Holdings
Date last seen Total Checkouts Price effective from Koha item type Lost status Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
07/03/2017   07/03/2017 Article Not Lost     Reprints Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 07/03/2017

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