Knowledge Center Catalog

Molecular characterization of a new waxy allele with partial expression in spelt wheat (Record no. 29354)

MARC details
000 -LEADER
fixed length control field 02405nab a22003977a 4500
001 - CONTROL NUMBER
control field G96930
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20190717174555.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 121211b |||p||p||||||| |z||| |
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 1432-2048 (Revista en electrónico)
022 0# - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 0032-0935
024 8# - OTHER STANDARD IDENTIFIER
Standard number or code https://doi.org/10.1007/s00425-011-1577-7
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title En
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) REP-13294
100 1# - MAIN ENTRY--PERSONAL NAME
9 (RLIN) 957
Personal name Guzman, C.
Miscellaneous information Global Wheat Program
Field link and sequence number INT3466
245 00 - TITLE STATEMENT
Title Molecular characterization of a new waxy allele with partial expression in spelt wheat
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Date of publication, distribution, etc. 2012
500 ## - GENERAL NOTE
General note Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0032-0935
520 ## - SUMMARY, ETC.
Summary, etc. Starch composition which is dependent on the waxy protein, the enzyme responsible for amylose synthesis in the grain, is an important aspect of the wheat quality. In this report, we describe the characterization of a novel Wx-A1 allele (Wx-A1g formerly known as -Wx-A1a) in Spanish spelt wheat lines which is responsible for a remarkable decline in the concentration of Wx-A1 protein found in the endosperm. Comparison of the DNA sequences in the Wx-A1a and Wx-A1g alleles showed the presence of a 160-bp insertion within the fourth intron in the latter. This insertion had some characteristics of a transposable-like element. RT-PCR analysis showed the presence of normal and aberrant mRNA transcripts in the Wx-A1g lines, indicating that the aberrant transcripts are un-spliced and contained the longer fourth intron. This may be related to the low level of Wx-A1 protein in these lines. In addition, a simple and fast PCR assay was designed for differentiating among different Wx-A1 alleles (a, b, f and g). The mutation described here is not related to either of the Wx-A1 mutations identified previously in common and durum wheats and could help to extend the range of amylose content of wheats.
536 ## - FUNDING INFORMATION NOTE
Text of note Global Wheat Program
546 ## - LANGUAGE NOTE
Language note English
591 ## - CATALOGING NOTES
Affiliation No CIMMYT affiliation
594 ## - STAFFID
StaffID INT3466
595 ## - COLLECTION
Collection Reprints Collection
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Null mutations
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Spelt wheat
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Starch
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Waxy proteins
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Alvarez, J.B.,
Relator term coaut.
9 (RLIN) 2175
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Caballero, L.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Yamamori, M.,
Relator term coaut.
9 (RLIN) 2176
773 0# - HOST ITEM ENTRY
Title Planta
Related parts v. 235, no. 6, p. 1331-1339
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Holdings
Date last seen Total Checkouts Full call number Price effective from Koha item type Lost status Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
07/03/2017   REP-13294 07/03/2017 Article Not Lost     Reprints Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 07/03/2017

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