Knowledge Center Catalog

Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction (Record no. 29063)

MARC details
000 -LEADER
fixed length control field 02213nab a22003017a 4500
001 - CONTROL NUMBER
control field G96416
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 121211b |||p||p||||||| |z||| |
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title En
043 ## - GEOGRAPHIC AREA CODE
Geographic area code US
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Versalovic, J.
245 00 - TITLE STATEMENT
Title Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Date of publication, distribution, etc. 1994
520 ## - SUMMARY, ETC.
Summary, etc. Interspersed repetitive sequences in prokaryotic genomes can be used as oligonucleotide primer binding sites for polymerase chain reaction (PCR)-mediated genomic fingerprinting (rep-PCR). rep-PCR is based on the observation that outwardly facing oligonucleotide primers, complementary to interspersed repeated sequences, enable the amplification of differently sized DNA fragments, consisting of sequences lying between these elements. Multiple amplicons of different sizes can be fractionated by electrophoresis and enable the establishment of DNA fingerprint patterns specific for individual bacterial strains. Several of these interspersed repetitive elements are conserved in diverse genera of bacteria and, therefore, enable single primer sets to be used for DNA fingerprinting many different microorganisms. Purified genomic DNA, crude bacterial cell lysates, or infected samples can be used directly in rep-PCR to generate DNA fingerprint profiles for comparative analyses. Detection and analysis of differently sized rep-PCR products include visual inspection of ethidium bromide-stained gels, computer-assisted detection and data storage methods, or laser scanning detection of fluorophore-labeled amplification products. DNA fingerprint patterns can be compared to estimate relative degrees of similarity between isolates and help determine whether isolates are clonally related.
546 ## - LANGUAGE NOTE
Language note English
595 ## - COLLECTION
Collection Reprints Collection
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Bacteria
9 (RLIN) 1017
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Dna fingerprinting
650 17 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Genomes
Miscellaneous information AGROVOC
Source of heading or term
9 (RLIN) 1131
650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Polymerase chain reaction
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Bruijn, F.J. de,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Lupski, J.R.,
Relator term coaut.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Schneider, M.,
Relator term coaut.
773 0# - HOST ITEM ENTRY
Title Methods in Molecular and Celluler Biology
Related parts v. 5, no. 1, p. 25-40
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Holdings
Date last seen Total Checkouts Price effective from Koha item type Lost status Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
07/03/2017   07/03/2017 Article Not Lost     Reprints Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 07/03/2017

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