Knowledge Center Catalog

Comparative analyses of LMW glutenin alleles in bread wheat using allele-specific PCR and SDS-PAGE (Record no. 28698)

MARC details
000 -LEADER
fixed length control field 02735nab a22003977a 4500
001 - CONTROL NUMBER
control field G95584
003 - CONTROL NUMBER IDENTIFIER
control field MX-TxCIM
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20230331215652.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 211019s2011 xxu|||p|op||| 00| 0 eng d
022 0# - INTERNATIONAL STANDARD SERIAL NUMBER
International Standard Serial Number 0733-5210
024 8# - OTHER STANDARD IDENTIFIER
Standard number or code https://doi.org/10.1016/j.jcs.2011.09.004
040 ## - CATALOGING SOURCE
Original cataloging agency MX-TxCIM
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) CIS-6395
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Ram, S.
9 (RLIN) 17641
245 10 - TITLE STATEMENT
Title Comparative analyses of LMW glutenin alleles in bread wheat using allele-specific PCR and SDS-PAGE
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. USA :
Name of publisher, distributor, etc. Elsevier,
Date of publication, distribution, etc. 2011.
500 ## - GENERAL NOTE
General note Peer review
500 ## - GENERAL NOTE
General note Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0733-5210
520 ## - SUMMARY, ETC.
Summary, etc. One hundred and eighty-two bread wheat cultivars developed in India were characterized for low molecular weight (LMW) glutenins using SDS-PAGE and allele-specific polymerase chain reaction (PCR) to assess allelic diversity encoded by Glu-3 loci, as well as their utility for correctly identifying different alleles. SDS-PAGE indicated Glu-A3c is present in 64.6% of the cultivars, Glu-A3b in 13.8%, Glu-A3d in 12.7% and Glu-A3e/f in 8.8%. Seven types of alleles were present at the Glu-B3 locus: Glu-B3b (29.3%), Glu-B3g (27.0%), Glu-B3h (13.8%), Glu-B3i (16.1%), Glu-B3j (12.1%), Glu-B3c (0.6%) and Glu-B3d (1.1%). SDS-PAGE found three types of Glu-D3 alleles: Glu-D3a (30.2%), Glu-D3b (67.1%) and Glu-D3c (2.7%). However, PCR found two different alleles in cultivars classified as carrying Glu-D3a and three alleles in those identified as carrying Glu-D3b cultivars, indicating a more complex nature of the Glu-D3 locus. In conclusion, the data found greater consistency between the SDS-PAGE and PCR amplification patterns of alleles such as Glu-A3c, Glu-A3d, Glu-B3g, Glu-B3h and Glu-B3i, and less consistency between those same patterns in the Glu-A3b, Glu-A3e/f and Glu-B3b alleles. More studies are needed in order to achieve unambiguous identification of the Glu-3 alleles and thereby allow their greater utility in germplasm evaluation and breeding.
536 ## - FUNDING INFORMATION NOTE
Text of note Global Wheat Program
546 ## - LANGUAGE NOTE
Language note Text in English
591 ## - CATALOGING NOTES
Affiliation CIMMYT Informa No. 1775|Elsevier
594 ## - STAFFID
StaffID INT0368
595 ## - COLLECTION
Collection CIMMYT Staff Publications Collection
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element PCR
Source of heading or term AGROVOC
9 (RLIN) 12563
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Wheat
Source of heading or term AGROVOC
9 (RLIN) 1310
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 1137
Topical term or geographic name as entry element Glutenins
Source of heading or term AGROVOC
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Sharma, S.
9 (RLIN) 24122
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Verma, A.
9 (RLIN) 24123
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Tyagi, B.S.
9 (RLIN) 10074
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Peña-Bautista, R.J.
Field link and sequence number INT0368
Miscellaneous information Global Wheat Program
9 (RLIN) 645
773 0# - HOST ITEM ENTRY
Title Journal of Cereal Science
Related parts v. 54, no. 3, p. 488-493
Place, publisher, and date of publication USA : Elsevier, 2011.
International Standard Serial Number 0733-5210
Record control number G444514
856 4# - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier https://hdl.handle.net/20.500.12665/1435
Link text Access only for CIMMYT Staff
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Article
Source of classification or shelving scheme Dewey Decimal Classification
Suppress in OPAC No
Holdings
Date last seen Total Checkouts Full call number Price effective from Koha item type Lost status Damaged status Not for loan Collection code Withdrawn status Home library Current library Date acquired
07/03/2017   CIS-6395 07/03/2017 Article Not Lost     CIMMYT Staff Publications Collection   CIMMYT Knowledge Center: John Woolston Library CIMMYT Knowledge Center: John Woolston Library 07/03/2017

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021.
Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
If you have any question, please contact us at
CIMMYT-Knowledge-Center@cgiar.org