MARC details
| 000 -LEADER |
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| fixed length control field |
02165nab a22003017a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
G95431 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20170719155424.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
121211b |||p||p||||||| |z||| | |
| 022 0# - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
0166-0934 |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
MX-TxCIM |
| 041 0# - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
En |
| 043 ## - GEOGRAPHIC AREA CODE |
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| Geographic area code |
US |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Deb, M. |
| 245 00 - TITLE STATEMENT |
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| Title |
Development of a multiplexed PCR detection method for barley and cereal yellow dwarf viruses, wheat spindle streak virus, wheat streak mosaic virus and soil-borne wheat mosaic virus |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Date of publication, distribution, etc. |
2008 |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Barley and Cereal yellow dwarf viruses (B/CYDVs), Wheat spindle streak mosaic (WSSMV), Soil-borne wheat mosaic virus (SBWMV) and Wheat streak mosaic virus (WSMV) constitute the most economically important group of wheat viruses. In this paper, a multiplex reverse transcription polymerase chain reaction (M-RT-PCR) method was developed for the simultaneous detection and discrimination of eight viruses: five strains of B/CYDVs, WSSMV, SBWMV and WSMV. The protocol uses specific primer sets for each virus producing five distinct fragments 295, 175, 400, 237, and 365 bp, indicating the presence of two strains of BYDVs,-PAV, -MAV, CYDV-RPV and two unassigned Luteoviridae BYDV-SGV and -RMV, respectively. This system also readily detected WSSMV, SBWMV and WSMV specific amplicons at 154, 219 and 193 bp, respectively. The amplification specificity of these primers was tested against a range of field samples from different parts of United States. The protocol also utilizes fluorescently tagged primers that can streamline the detection of each virus through capillary electrophoresis. This study fulfills the need for a rapid and specific wheat virus diagnostic tool that also has the potential for investigating the epidemiology of these viral diseases. |
| 546 ## - LANGUAGE NOTE |
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| Language note |
English |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Barley yellow dwarf virus |
| 9 (RLIN) |
1019 |
| 650 10 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
multiplex PCR |
| 653 0# - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Cereal yellow dwarf virus |
| 653 0# - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Soil borne wheat mosaic virus |
| 653 0# - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Wheat streak mosaic virus |
| 653 0# - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Wheat viruses |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Anderson, J.M., |
| Relator term |
coaut. |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Journal of Virological Methods |
| Related parts |
v. 148, p. 17-24 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Article |
| Source of classification or shelving scheme |
Dewey Decimal Classification |